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Rice early-heading main-effect QTL molecular markers, identifying method thereof, and applications of molecular markers and identifying method

A technology of molecular markers and identification methods, applied in the field of molecular biology, can solve problems such as the inability to apply new varieties of early-maturing rice varieties and differences in regulatory mechanisms, and achieve the effects of facilitating hybridization, speeding up cloning, and enriching knowledge and understanding

Active Publication Date: 2017-02-15
GUANGXI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many genes homologous to Arabidopsis flowering genes have been cloned in rice, there are great differences in their regulatory mechanisms for flowering; Overexpression of heading genes can also lead to early heading characteristics in rice, but due to differences in genetic backgrounds, these homologous genes have not yet been applied to the breeding process of new early-maturing rice varieties

Method used

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  • Rice early-heading main-effect QTL molecular markers, identifying method thereof, and applications of molecular markers and identifying method
  • Rice early-heading main-effect QTL molecular markers, identifying method thereof, and applications of molecular markers and identifying method
  • Rice early-heading main-effect QTL molecular markers, identifying method thereof, and applications of molecular markers and identifying method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Screening and positioning of molecular markers

[0050] (1) Construction of early heading chromosome segment substitution line CSSL13 and analysis of substitutional segments

[0051] Using 9311 as the recipient parent and Guangxi common wild rice DP30 as the donor parent, through continuous backcrossing, selfing and directional selection, the BC 4 f 2 The stable genetic early heading chromosome segment substitution line CSSL13 was obtained from this generation.

[0052] According to the SSR marker published on the gramene website (http: / / www.gramene.org / ) and the STS marker designed by the rice indica stalk sequence difference, the polymorphism between the parents DP30 and 9311 was detected, and a total of 408 pairs showing The 408 pairs of polymorphic molecular markers were used to analyze the substitution fragments of CSSL13. The results showed that CSSL13 contained 5 substitution fragments of the donor parent DP30, which were located at the 1st, 2nd, 3rd...

Embodiment 2

[0081] Embodiment 2 Molecular marker identification method

[0082] Using the whole genome DNA of the rice material to be identified as a template, use one or more pairs of primers corresponding to the above-mentioned molecular markers RM6670, M55, M07, M13 and M15 to carry out PCR amplification, and detect the amplified products.

[0083] (1) The donor parent, recipient parent and F were extracted by conventional CTAB method 2 Separate the DNA of each individual plant in the population;

[0084] (2) The reaction system of PCR is as follows:

[0085]

[0086] The PCR reaction conditions were: denaturation at 94°C for 5 min, 30 s at 94°C, 30 s at 56°C, 60 s at 72°C, 35 cycles of amplification, and final extension at 72°C for 10 min.

[0087] The DNA amplification products were detected by polyacrylamide gel electrophoresis, and the amplified DNA bands were recorded by silver staining.

[0088] (3) Result analysis

[0089](1) Use molecular marker RM6670 primers to amplify...

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Abstract

The invention discloses rice early-heading main-effect QTL molecular markers, an identifying method of the rice early-heading main-effect QTL molecular markers, and applications of the molecular markers and the identifying method. The found rice early-heading main-effect QTL qHD8.3 molecular markers are respectively RM6670, M55, M07, M13 and M15, one pair or a plurality of pairs of primers corresponding to the abovementioned molecular markers are adopted for carrying out PCR amplification, and the amplification product is detected. The invention further provides applications of the molecular markers or the identifying method of the molecular markers in locating and cloning of rice early-heading QTL qHD8.3. With the adoption of the technical scheme, the cloning of the early-heading QTL qHD8.3 can be accelerated, the technology can be applied to the assisted selective breeding, the timely hybridization trans-breeding process is facilitated, and finally, the breeding process is accelerated.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a rice early heading main effect QTL molecular marker and its identification method and application. Background technique [0002] As one of the most important food crops in my country and even in the world, rice production is of great strategic significance for solving future global food problems. However, most of the existing rice varieties have strong regional and seasonal limitations, which largely affect the further improvement of important agronomic traits such as yield and quality. Therefore, improving the regional and seasonal adaptability of rice varieties and cultivating New varieties of rice that adapt to the ecological environment and cultivation system of a specific area have become one of the main goals of rice breeding today. [0003] Heading date is an important agronomic trait that determines the regional and seasonal adaptability of rice varieties, plays an imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 覃宝祥李容柏韦敏益吴子帅刘立龙刘芳罗继景邱永福
Owner GUANGXI UNIV
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