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Enzyme preparation for tobacco processing and application of enzyme preparation

A technology of enzyme preparation and tobacco leaves, which is applied in the field of reducing miscellaneous flavors of tobacco leaves, can solve the problems of long storage period, large backlog of funds, and low production cost, and achieve the effects of improving tobacco miscellaneous flavors, good application prospects, and simple process

Active Publication Date: 2017-02-15
CHINA TOBACCO HEBEI INDUSTRIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of natural aging tobacco leaves are long storage period (generally 1 to 3 years), large warehouse occupation, large backlog of funds, and high production costs. However, due to natural aging, tobacco leaves have bright color, beautiful aroma, weak irritation and simple process. , easy operation and other advantages, and the effect of improving the aroma and taste quality is obviously better than that of artificially aged tobacco leaves. Therefore, the method of natural aging is generally adopted in economically developed countries.
However, the artificial aging period is short (generally 12-20 days), the backlog of funds is small, and the production cost is low, but the quality of tobacco leaves is not as good as that of natural aging.
Restricted by economic conditions in our country, domestic small and medium-sized cigarette factories still mainly use artificial aging. Even large-scale cigarette factories also face the problem of reducing production costs and improving economic benefits while improving product quality, so they cannot completely get rid of artificial aging. change

Method used

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  • Enzyme preparation for tobacco processing and application of enzyme preparation
  • Enzyme preparation for tobacco processing and application of enzyme preparation
  • Enzyme preparation for tobacco processing and application of enzyme preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～8

[0032] The sensory evaluation of the tobacco leaf group after the treatment of the enzyme preparation in Examples 1-8

[0033] Embodiments 1 to 8 select different tobacco leaf groups (see Table 2) respectively, select different enzyme formulations (see Table 3) for the characteristics of each leaf group, and treat 8 different types of tobacco leaves according to the process parameters in Table 4. Leaves were treated with enzymes.

[0034] The tobacco leaf group that uses in the embodiment 1~8 of table 2

[0035]

[0036]

[0037] Composition and application rate (enzyme activity unit / gram tobacco leaf) of enzyme preparation in table 3 embodiment 1~8

[0038] Example 1 2 3 4 5 6 7 8 α-amylase 6 15 23 39 80 121 177 27 β-amylase 9 18 2 22 14 0 160 12 Glucoamylase 96 137 111 169 600 459 485 189 Pullulanase 9 130 151 168 5 169 320 6 cellulase 160 190 152 88 5 175 23 23 hemicellulase 199 ...

Embodiment 9

[0046] Embodiment 9 enzyme activity assay method

[0047] (1) α-amylase: Add 1 mL of 0.5% soluble starch solution and 2.5 mL of disodium hydrogen phosphate-citric acid buffer solution at pH 4.2 to a 10 mL test tube successively, and preheat at 40° C. for 10 min. Then add 0.5mL enzyme solution diluted multiple times, after 5min at 40°C, add 0.1mol / L H 2 SO 4 1 mL to stop the reaction. Then take 2mL of reaction solution and 0.5mL of 0.5% KI-I2 solution to develop color, and measure OD 620 . After the starch concentration of the reaction solution was compared with the starch gradient standard curve, the enzyme activity was calculated.

[0048] At pH 4.2 and temperature 40°C, the amount of enzyme required to hydrolyze 1 mg of soluble starch in 5 minutes is one unit of enzyme activity.

[0049] (2) β-amylase: Add 1 mL of 0.5% soluble starch solution and 2.5 mL of disodium hydrogen phosphate-citric acid buffer solution at pH 4.2 to a 10 mL test tube successively, and preheat a...

Embodiment 10

[0068] Chemical composition analysis of tobacco leaf group after embodiment 10 enzyme treatment

[0069] Get the tobacco leaf group after enzyme treatment in embodiment 2 and embodiment 3, have carried out the analysis of shredded tobacco chemical composition with pure water treatment contrast sample, have detected the content of starch, reducing sugar, total protein, the result is as follows figure 2 shown.

[0070] The chemical composition analysis results show that the enzyme preparation treatment of the present invention can significantly reduce the content of macromolecular compounds harmful to tobacco smoking quality, thereby improving the quality of tobacco leaves.

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Abstract

The invention provides an enzyme preparation and a method for improving foreign flavor of tobacco leaves and fragrance by utilizing the enzyme preparation in a tobacco primary processing process. Through treatment of the enzyme preparation, macromolecular compounds harmful to tobacco smoking quality can be remarkably degraded, so that the effects of improving the foreign flavor of the tobacco leaves and the fragrance are achieved.

Description

technical field [0001] The invention relates to the technical field of tobacco shredded processing, in particular to an enzyme preparation used in the tobacco shredded processing technology, and a method for reducing the miscellaneous taste of tobacco leaves and improving the aroma of tobacco leaves by adding the enzyme preparation. Background technique [0002] In tobacco products, the aroma and taste of tobacco are crucial. [0003] Tobacco leaves have many and complex chemical components, among which macromolecular compounds such as protein, starch and cell wall substances account for the largest proportion. These macromolecular compounds not only have side effects on tobacco smoking, but also are the precursors of most harmful components of smoke. [0004] Among the many components of tobacco leaves, the protein content is the most abundant. For example, the protein content in Burley tobacco is as high as 20.48%. The protein in tobacco leaves is divided into soluble pr...

Claims

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Application Information

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IPC IPC(8): C12N9/26C12N9/02C12N9/42C12N9/44C12N9/50C12N9/64C12N9/88C12N9/76C12N9/20A24B3/12A24B3/04A24B15/18
CPCA24B3/04A24B3/12A24B15/18C12N9/0061C12N9/20C12N9/2402C12N9/2414C12N9/2425C12N9/2428C12N9/2437C12N9/2457C12N9/2477C12N9/248C12N9/50C12N9/63C12N9/6427C12N9/6481C12N9/88C12Y110/03002C12Y301/01011C12Y302/01001C12Y302/01002C12Y302/01003C12Y302/01015C12Y302/01041C12Y304/21004C12Y304/22002C12Y304/22003C12Y304/22004C12Y304/22031C12Y402/02002
Inventor 戴光解民王尧
Owner CHINA TOBACCO HEBEI INDUSTRIAL CO LTD
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