Primary culture shoot induction method for lithocarpus elmerrillii

A technology of primary culture and bud induction, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as browning of explants, and achieve the effects of improving germination rate, overcoming vitrification, and reducing pollution rate.

Inactive Publication Date: 2017-02-15
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method that can overcome the problems of explant browning and vitrification in the bud induction process, improve the bud induction rate, bud index and bud growth status, and obtain a large number of buds with good quality, thereby satisfying the requirements of proliferation and cultivation. Need, promote the Wanningke primary culture bud induction method for the establishment of the rapid propagation system of Wanningke tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The semi-lignified shoots of Wanningke germinated from the base, with a length of 6-7 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of Wanning Kemengtiao, put it in a triangular flask, add a solution of detergent with a volume concentration of 1%, seal the bottle mouth with gauze, and wash it on a shaking table with a rotation speed of 200 r / min for 10 min After that, rinse them under running water for 2 h, then wash them twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse them with sterile water for 8 min. After ~10 times, cut the 3.5 cm long stem segments and inoculate them in the bud induction medium, and inoculate 2 stem segments in each bottle.

[0022] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-KT 1.0 mg / L + vitamin C 10 mg / L + lactose 5.0 g / L + sucrose 20.0 g / L. Wherein the imp...

Embodiment 2

[0025] The semi-lignified shoots of Wanningke germinated from the base, with a length of 7-8 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of Wanning Kemengtiao, put them in a triangular flask, add a 2% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it on a shaker at 200 r / min for 10 minutes After that, rinse under running water for 1.5 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8 After ~10 times, cut the 3.5 cm long stem segments and inoculate them in the bud induction medium, and inoculate 2 stem segments in each bottle.

[0026] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-KT 1.5 mg / L + vitamin C 10 mg / L + lactose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to weight r...

Embodiment 3

[0029] The semi-lignified shoots of Wanningke germinated from the base, with a length of 7-8 cm and a diameter of 0.3-0.4 cm were selected as explants. Cut off the needles and leaves of Wanning Kemengtiao, put them in a triangular flask, add a solution of detergent with a volume concentration of 4%, seal the mouth of the bottle with gauze, and wash it on a shaker at a speed of 200 r / min for 15 minutes After that, rinse under running water for 1 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8 After ~10 times, the 4.0 cm long stem segments were inoculated in the bud induction medium, and 2 stem segments were inoculated in each bottle.

[0030] The raw material content of the bud induction medium is: 1 / 2 improved WPM medium + 6-KT 1.5 mg / L + vitamin C 10 mg / L + lactose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium compositi...

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PUM

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Abstract

The invention discloses a primary culture shoot induction method for lithocarpus elmerrillii. The method is characterized in that a basal coppice shoot of lithocarpus elmerrillii is taken as explant and subjected to aseptic treatment, a high-quality primary culture shoot induction medium is inoculated with the explant, the explant is cultured under the conditions of proper temperature, humidity and illumination intensity for induction of germination. With the adoption of the method, the problems of browning, vitrification and the like of the explant during shoot induction are solved, the shoot induction rate, the germination index and the shoot growth condition are increased, a large number of shoots with good quality are obtained, the enrichment culture requirement is met, and construction of a rapid propagation system for tissue culture of lithocarpus elmerrillii is promoted.

Description

technical field [0001] The invention relates to Wanningke asexual reproduction technology, in particular to a method for inducing Wanningke primary cultured buds. Background technique [0002] Wanningke (Lithocarpus elmerrillii), is an arbor of the genus Lithocarpus in the Fagaceae (Fagaceae), up to 25 meters, the current year branches have longitudinal grooves, glabrous, dark brown after drying, and the 2 or 3-year-old branches are scattered with off-white bark Pores, bud scales semicircular or broadly triangular, oily and shiny when dry. Leaf thin leathery, long elliptic or rarely obovate elliptic, 10-17cm long, 3-6cm wide, apex acuminate, base attenuate long, extending down to base along petiole, entire, lateral Veins 9-12 on each side, sharply curved upward near the leaf margin and submerged, branch veins are slender, with a dense wax scale layer on the back of the leaf, grayish white after drying; petiole 2-2.5 cm long. Female flowers are solitary and scattered on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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