Manufacturing and application method of bioenzyme-process keratinization mask
A technology of biological enzyme method and production method, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of leading enterprises without representative significance, and achieve high safety effects
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[0019] The present invention is further described in detail below.
[0020] A method for making and using a bio-enzymatic exfoliating facial mask, characterized in that:
[0021] The casein material is pulverized, sterilized by high-pressure steam for 30 minutes, and then baked in an oven at 80°C for 12 hours to constant weight;
[0022] Keratinase-producing bacteria were inoculated in 100ml LB medium (Luria-Bertani medium) according to 1% inoculum amount, 160rpm / min (rotation speed / minute), and activated by shaking culture for 24 hours; then inoculated into 150ml LB ( Luria-Bertani medium) culture medium, 160 rpm, about 3-4 hours, shaking culture to logarithmic phase (OD6 00 0.4 ~ 0.6, OD: optical density) to obtain keratinase-producing bacterial inoculum;
[0023] 5 L fermenter to produce keratinase, liquid volume 3.5 L, 52.5 g of materials rich in keratinized protein such as pre-treated feathers, hair, keratinized skin or casein, K 2 HPO 4 4.9 g, KH 2 PO 4 2.45 g, N...
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