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A kind of high-density fermentation medium and fermentation method thereof for feeding Lactobacillus brevis

A high-density fermentation, Lactobacillus brevis technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem of inhibiting the growth and reproduction of bacteria, consuming nutrient substrates, and requiring higher nutrient substances and culture conditions. It can prolong the logarithmic phase of bacterial growth, reduce production costs, and relieve the effect of feedback inhibition.

Active Publication Date: 2019-10-11
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that lactic acid bacteria have high requirements on nutrients and culture conditions, and continuously consume nutrient substrates and produce metabolites such as organic acids during the fermentation process, which inhibits the growth and reproduction of bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the high-density fermentation medium of Lactobacillus brevis ZLB004 and fermentation comparison

[0034] 1. The components and content of the high-density fermentation medium of Lactobacillus brevis ZLB004 are:

[0035] Glucose 30g, beef peptone 30g, beef extract 9g, yeast extract powder 3.5g, sodium acetate 4g, K 2 PO 4 ·3H 2 O 1g, diammonium hydrogen citrate 1g, ZnSO 4 ·7H 2 O 0.3g, MnSO 4 ·H 2 O 0.1g, Tween-80 1mL, distilled water 1000mL.

[0036] 2. Comparison between high-density fermentation and conventional fermentation

[0037] 2.1 The main steps of high-density fermentation are as follows

[0038] (1) Inoculate the Lactobacillus brevis ZLB004 strain preserved at -80°C in the MRS liquid medium, and after culturing at 37°C for 24 hours, transfer to the MRS liquid medium with 1% inoculum amount, culture for 18 hours, and passage like this After culturing twice, the activated strains were obtained;

[0039] (2) Prepare the components of the ...

Embodiment 2

[0049] Embodiment 2: the high-density fermentation medium of Lactobacillus brevis ZLB004 and fermentation comparison

[0050] 1. The components and content of the high-density fermentation medium of Lactobacillus brevis ZLB004 are:

[0051] Glucose 40g, beef peptone 40g, beef extract 10g, yeast extract powder 4.5g, sodium acetate 6g, K 2 PO 4 ·3H 2 O3g, diammonium hydrogen citrate 3g, ZnSO 4 ·7H 2 O 0.5g, MnSO 4 ·H 2 O 0.3g, Tween-80 1mL, distilled water 1000mL.

[0052] 2. Comparison between high-density fermentation and conventional fermentation

[0053] 2.1 The main steps of high-density fermentation are as follows

[0054] (1) Inoculate the Lactobacillus brevis ZLB004 strain preserved at -80°C in the MRS liquid medium, and after culturing at 37°C for 24 hours, transfer it to the MRS liquid medium with 1% inoculum amount, and cultivate it for 24 hours, so that it can be subcultured After culturing twice, the activated strains were obtained;

[0055] (2) Prepare th...

Embodiment 3

[0065] Embodiment 3: the high-density fermentation medium of Lactobacillus brevis ZLB004 and fermentation comparison

[0066] 1. The components and content of the high-density fermentation medium of Lactobacillus brevis ZLB004 are:

[0067] Glucose 35g, beef peptone 35g, beef extract 9.5g, yeast extract powder 4g, sodium acetate 5g, K 2 PO 4 ·3H 2 O 2g, diammonium hydrogen citrate 2g, ZnSO 4 ·7H 2 O 0.4g, MnSO 4 ·H 2 O 0.2g, Tween-80 1mL, distilled water 1000mL.

[0068] 2. Comparison between high-density fermentation and conventional fermentation

[0069] 2.1 The main steps of high-density fermentation are as follows

[0070] (1) Inoculate the Lactobacillus brevis ZLB004 strain preserved at -80°C in the MRS liquid medium, and after culturing at 37°C for 24 hours, transfer it to the MRS liquid medium with 1% inoculum amount, and cultivate it for 21 hours, so that it can be subcultured After culturing twice, the activated strains were obtained;

[0071] (2) Prepare th...

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PUM

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Abstract

The invention provides a high-density fermentation culture medium for feed lactobacillus brevis and a fermentation method thereof, and belongs to the field of fermentation of probiotics. The high-density fermentation culture medium has the advantages that for the lactobacillus brevis ZLB004, under the conditions of optimum high-density fermentation culture medium and fermentation, a constant-pH (potential of hydrogen) glucose supplementing and adding control strategy is adopted, and the number of living bacteria in fermentation liquid is more than 1010CFU / mL, and is increased by more than 20 times in comparison with the number of living bacteria cultured by the common MRS (Man, Rogosa and Sharpe) culture medium and conventional method; the high-density fermentation of lactobacillus brevis is realized, the culture volume is reduced, the production cost is reduced, and the production efficiency is improved.

Description

technical field [0001] The invention relates to a high-density fermentation medium of Lactobacillus brevis for feeding and a fermentation process thereof, belonging to the field of probiotic fermentation. Background technique [0002] Lactobacillus brevis (Lactobacillus brevis) is a beneficial lactobacillus commonly found in human and animal intestines, and is also one of the important populations of the genus Lactobacillus. It plays an important role in maintaining the micro-ecological balance of the gastrointestinal tract, promoting nutrient digestion and absorption, inhibiting pathogenic bacteria infection and immune regulation, and improving animal growth performance. Lactobacillus brevis ZLB004 (L.brevis ZLB004) is an excellent probiotic isolated from the intestinal mucosa of healthy weaned piglets. It has good acid resistance and bile salt tolerance. It has good effects in improving intestinal flora and improving body immunity (Liu et al., 2013, 2015; liu et al., 2015...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/24
Inventor 刘辉季海峰张董燕王四新张伟王晶王雅民
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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