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A method for directional rapid screening and enrichment of broad-spectrum nitrifying bacteria

A nitrifying bacteria, broad-spectrum technology, applied in the direction of bacteria, can solve the problems of poor adaptability, difficulty in forming ecological advantages, etc., and achieve the effect of NOB growth promotion

Active Publication Date: 2020-01-24
BEIJING UNIV OF TECH
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AI Technical Summary

Problems solved by technology

The problem with this technical route is that a single strain has poor adaptability to the environment
It is difficult for a single species of high-efficiency nitrifying bacteria to form an ecological advantage in a complex sewage treatment system where many types of bacteria exist

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This cultivation method has passed a small test, and the effect is remarkable. The specific operation is as follows:

[0021] (1) Use barrel SBR reactor to cultivate return sludge, carry out continuous aeration for 4 days, and do not add ammonia nitrogen, the original sludge concentration (MLSS) is 6976mg / L, DO is 1.5-2.0mg / L , COD was 16.084mg / L, MLSS changed to 5324mg / L after 4 days, DO content rose to 6.34mg / L, COD fluctuated up and down after a straight rise, and finally stabilized at around 60mg / L. The apparent conditions showed that a large number of heterotrophic bacteria were completely consumed, and then entered the stage of enriching broad-spectrum nitrifying bacteria.

[0022] (2) On the basis of step (1), adopt the method of intermittent water inflow, by controlling the influent water composition, the temperature in the system is 25°C, the DO is between 1.0mg / L and 1.2mg / L, and the pH is 7.5~ 8. Provide the best conditions for the growth of nitrifying bacte...

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Abstract

The invention relates to a method for directionally and fast screening enriched broad-spectrum nitrifying bacteria, and belongs to the field of water treatment. Activated sludge is used as a seed source; the structural characters of original AOB (ammonia oxidizing bacteria) and NOB (nitrite oxidizing bacteria) floras are maintained; a high-DO (dissolved oxygen) maintaining method is used; high efficiency of nitration is maintained; under the condition of high DO, FA (free ammonia) and FNA (free nitrous acid) are controlled to be lower than the inhibition concentration on the nitrifying bacteria by pH according to the concentration of ammonia nitrogen and nitrite; high nitrifying efficiency is maintained; the fast culture of the nitrifying bacteria is realized.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to directional rapid screening and enrichment of broad-spectrum nitrifying bacteria. The technology aims to quickly enrich and cultivate broad-spectrum nitrifying bacteria in a short period of time, including ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria. (NOB), the cultivation of this bacterial group has broad application prospects for the production of nitrifying bioactive fillers and the improvement of ammonia nitrogen nitrification performance in sewage plants. Background technique [0002] With the modernization of cities, the development of industry and agriculture, the pollution of nitrogen-containing wastewater is becoming increasingly serious. Excessive ammonia pollution will lead to eutrophication of water bodies, causing algae to proliferate in large numbers, thereby endangering aquatic organisms. Even destroy the ecological balance. Reducing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 杨宏张帆于濛雨刘毅田玉斌石欢李佳
Owner BEIJING UNIV OF TECH
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