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Effective parts of skyblue adonis and preparation method and application thereof

A technology for the side marigolds and effective parts of blue flower, applied in the field of plant extracts, can solve the problems that have not yet been seen, and achieve the effects of convenient extraction, high extraction efficiency, and improved extraction efficiency.

Inactive Publication Date: 2017-01-25
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research and application of calendula officinale to inhibit the activity of HDAC1 enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiments 1 to 3 are specific implementations of the effective parts of Calendula officinalis and its preparation method and application according to the present invention, wherein embodiment 1 is the best embodiment.

[0024] Example 1

[0025] The preparation method comprises the following steps: pulverize the whole calendula officinale herb, use an ethanol solution with a mass percentage concentration of 75 to 85% for ultrasonic extraction for 1 hour, then filter and reflux the extraction to obtain an extract, combine the extracts, concentrate and dry to obtain an infusion Extract; disperse the extract with distilled water to obtain an extract dispersion, then use sherwood oil, ethyl acetate and n-butanol as solvents to extract the extract dispersion, evaporate the solvent to obtain the indigo side calendula petroleum ether part, indigo The ethyl acetate part of Calendula officinalis and the n-butanol part of Calendula officinalis; the remaining extract dispersion ...

Embodiment 2

[0027] The preparation method comprises the following steps: pulverize the whole calendula officinale herb, use an ethanol solution with a mass percentage concentration of 85-95% for ultrasonic extraction for 3 hours, then filter, reflux and extract the filter residue twice, each time for 1 hour, and combine the extracts , concentrating and drying to obtain medicinal extract; adding distilled water to disperse medicinal extract to obtain medicinal extract dispersion liquid, then adopt sherwood oil, ethyl acetate and n-butanol successively as solvent extraction medicinal extract dispersion liquid, after waving off solvent, obtain blue-flowered calendula officinalis Petroleum ether part, Ethyl acetate part of Calendula officinalis and n-butanol part of Calendula officinalis; Concentrate and dry the remaining extract dispersion after extraction to obtain the aqueous phase extraction part of Calendula officinalis.

Embodiment 3

[0029] The preparation method comprises the following steps: pulverize the whole calendula officinale plant, extract it by ultrasonic wave with an ethanol solution with a mass percentage concentration of 65-75% for 0.5 hours, then filter, and extract the filter residue by reflux once for 2 hours, combine the extracts, Concentrate and dry to obtain the extract; add distilled water to disperse the extract to obtain the extract dispersion, then use petroleum ether, ethyl acetate and n-butanol as solvents to extract the extract dispersion, and evaporate the solvent to obtain the calendula officinalis oil The ether part, the ethyl acetate part of Calendula indigo and the n-butanol part of Calendula indigo; the remaining extract dispersion after extraction is concentrated and dried to obtain the aqueous phase extraction part of Calendula indigo.

[0030] Performance Testing

[0031] Respectively take 0.4 mg of the dried indigo calendula petroleum ether fraction, indigo calendula eth...

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PUM

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Abstract

The invention discloses the effective parts of skyblue adonis and the preparation method and application thereof and belongs to the technical field of plant extract. The method comprises of the steps of selecting the mixture of one or more than two extracts from the petroleum ether part, ethyl acetate, normal butyl alcohol part and water phase extraction part of skyblue adonis. Crushing the skyblue adonis and adding 65%-95% (weight) ethanol solution for extraction, concentrating and drying the extracting solution to obtain the extract; adding distilled water for dispersion to obtain extract dispersion, then respectively using petroleum ether, ethyl acetate and normal butyl alcohol as the solvent to extract the extract dispersion, making the solvent volatilize to obtain the petroleum ether part, ethyl acetate, normal butyl alcohol part and water phase extraction part of skyblue adonis; concentrating and drying the extracted extract dispersion to obtain the water phase extraction part of skyblue adonis. The effective parts of skyblue adonis of the invention can be used to prepare drugs inhibiting HDAC1 enzyme, and prepare cancer resistant drugs.

Description

technical field [0001] The invention relates to effective parts of Calendula officinalis and its preparation method and application, and belongs to the technical field of plant extracts. Background technique [0002] Adonis coerulea Maxim is a perennial herb of the family Ranunculaceae and is 3-15 cm high. It is distributed in alpine grasslands or shrubs at an altitude of 3350-5200m in my country's Tibet, Sichuan, Qinghai and Gansu. It is a Tibetan medicine. The medicinal materials widely used in the system for the treatment of sores and psoriasis [See: Chinese Academy of Sciences Chinese Flora Editorial Committee. Chinese Flora [M]. Beijing Science Press, 1980.]. "Tibetan Medicine Annals" records that the medicinal whole plant of Calendula officinalis is "bitter, cold, and externally used to treat sores, boils, carbuncles, herpes, scabies and psoriasis" [see: Yang Yongchang, Tibetan Medicine Annals [M], Qinghai People's Publishing House, 1991]. The related activity of Adon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P35/00
CPCA61K36/71A61K2236/333A61K2236/39A61K2236/55
Inventor 芦永昌吴疆林鹏程钱帅白雪李海丽郑虹朱奎德曾擎屹
Owner QINGHAI UNIV FOR NATITIES
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