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GC-MS coupling technology-based method for analysis of components in cockroaches in different growing stages

A combined technology and GC-MS technology, which is applied in the field of analysis of components in cockroaches, can solve problems such as the small number and types of components and the lack of analysis and research on cockroaches.

Inactive Publication Date: 2017-01-18
SICHUAN GOODDOCTOR PANXI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, GC-MS is used to study the fat-soluble components of cockroaches. As a result, the identified components are less in quantity and type, and the research objects are limited to adults of cockroaches. Lack of analysis and research

Method used

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  • GC-MS coupling technology-based method for analysis of components in cockroaches in different growing stages
  • GC-MS coupling technology-based method for analysis of components in cockroaches in different growing stages
  • GC-MS coupling technology-based method for analysis of components in cockroaches in different growing stages

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Experimental program
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Embodiment 1

[0014] 1 Experimental instruments, materials and reagents

[0015] Materials: Dried bodies of 3-4 instar nymphs, 7-8 instar nymphs, newly moulted adults, male adults and female adults of cockroaches, provided by Sichuan Good Doctor Pharmaceutical Group Co., Ltd. All reagents used were of analytical grade.

[0016] Instruments: GC-MS instrument (Shimadzu GCMS-QP2010, Japan), rotary evaporator (Shanghai Yarong Biochemical Instrument Factory RE-5298), drying oven (Shanghai Yiheng Science Co., Ltd. DHG-9140), ultrasonic instrument (USA SONICS VCX150), pulverizer (Shanghai Gaoxiang Food Machinery Factory GX-02).

[0017] 2 Experimental part

[0018] 2.1 Ethanol extraction

[0019] Crush the dried whole cockroaches in different states, weigh 5g, add 15 times the amount of 95% ethanol, soak overnight at room temperature, extract 3 times by ultrasonic extraction at 65°C for 30 minutes, and combine the filtrates for use after suction filtration The solvent was removed by a rotary e...

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Abstract

The invention provides a method for identification and analysis of components in cockroaches, comprising the following specific steps: (1) collecting cockroach samples in five growing stages of male adults, female adults, ecdysis adults, 7th-8th instar nymphs and 3nd-4th instar nymphs; (2) preparing the cockroach samples in different growing stages: extracting with 95% ethanol; (3) optimizing GC-MS coupling analysis conditions: optimizing GC-MS analysis conditions of components in cockroaches in different growing stages by selection of sample port temperature, sample size and sample mode; and (4) identifying specific components of cockroaches in each growing stage. The method of the invention has high sensitivity and reliable identification results, and can be used for identification of cockroaches in different growing stages. By the method, research and utilization of components of cockroaches in each growing stage are enhanced.

Description

technical field [0001] The invention relates to a method for analyzing components in cockroaches, in particular to a method for analyzing fat-soluble components in cockroaches based on GC-MS combined technology. Background technique [0002] Cockroaches, belonging to the class Insecta, commonly known as cockroaches, are one of the oldest insects in the world and the most primitive winged insects in existence. The adult body is about 30mm long, the body is flat and round, the head is small, the body color is reddish brown, the pronotum is trapezoidal, the middle part and the rear edge are reddish brown, the rest is light yellow, and the edge is a circle of yellow Band, with a brown butterfly spot in the center of the front. The harvest objects of cockroaches are usually adults, because of their slow growth, it takes a long time and a high cost to raise them to adults. [0003] In the prior art, GC-MS is used to study the fat-soluble components of cockroaches. As a result, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 耿福能刘越飞耿福昌
Owner SICHUAN GOODDOCTOR PANXI PHARMA
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