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Preparation method of gold nanocluster fluorescent system based on surface plasmon enhancement

A surface plasmon and gold nanocluster technology, applied in the field of gold nanoclusters, can solve the problems of limiting the application of AuNCs, low luminescence quantum yield, biocompatibility, toxicity and low photostability, etc.

Active Publication Date: 2018-05-01
天津市源创环保设备有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Compared with traditional fluorescent markers, AuNCs have many advantages, such as: high biocompatibility, low toxicity, photostability, can be synthesized under very mild conditions, and the luminescence color varies with The size of the cluster is adjustable; but it has a fatal defect, that is, the luminescence quantum yield is low, usually lower than 10%. It is this defect that greatly limits the application of AuNCs

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  • Preparation method of gold nanocluster fluorescent system based on surface plasmon enhancement
  • Preparation method of gold nanocluster fluorescent system based on surface plasmon enhancement
  • Preparation method of gold nanocluster fluorescent system based on surface plasmon enhancement

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preparation example Construction

[0028] The invention provides a method for preparing a gold nanocluster fluorescent system based on surface plasmon enhancement, comprising: contacting a gold nanocluster solution, a modified gold nanorod solution and a buffer solution to obtain a surface plasmon-based fluorescent system. Enhanced gold nanocluster fluorescence system;

[0029] Wherein, the modified gold nanorods in the modified gold nanorod solution include gold nanorods, a silicon dioxide layer and an amino-modified layer, the silicon dioxide layer is coated on the outside of the gold nanorods, and the amino-modified layer is distributed on the silicon dioxide The outer surface of the layer; the outer protective group of the gold nanocluster in the gold nanocluster solution is selected from one or more of bovine serum albumin, human serum albumin and glutathione.

[0030] In the present invention, the amount of gold nanoclusters and modified gold nanorods can be selected in a wide range, but in order to furth...

preparation example 1

[0046] Preparation of gold nanorod (AuNR) solution:

[0047] 1) Preparation of gold seeds: Weigh 0.3645g of CTAB (cetyltrimethylammonium bromide) into a 50mL Erlenmeyer flask, add 10mL of secondary water to dissolve, and add 250μL of 0.01M HAuCl 4 solution and 600 μL of NaBH 4 The solution (prepared in ice water) was quickly shaken for 2 minutes, and the color of the solution changed from yellow to light brown. The solution was placed in a water bath at 30°C for 2 hours to prepare a gold seed solution.

[0048] 2) Preparation of growth solution: Weigh 5.4675g of CTAB into a 250mL volumetric flask, add 150mL of secondary water to dissolve, then add 7.5mL of 0.01M HAuCl 4 , shake well and add 1.2mL of 0.01M AgNO 3 solution; shake well, add 3mL of 1M HCl, shake well, add 1.2mL of 0.1M AA (ascorbic acid) to prepare growth solution.

[0049] 3) After mixing and shaking the growth solution evenly, take 210 μL of the seed solution and quickly inject it into the above gold seed so...

preparation example 2

[0052] AuNR@SiO 2 @NH 2 Solution preparation:

[0053] 1) AuNR@SiO 2 Preparation of the solution: Take 20 mL of the above-mentioned purified gold nanorod solution and place it in a 50 mL round bottom flask, adjust the pH to 10-11 with 0.1 M NaOH solution, and after stirring for 1 h, add 10 μL of 20% by weight gold every 30 min. The methanol solution of TEOS (added three times, a total of 30 μL) was stirred at 25° C. for 36 h. Take 14ml of solution and wash it three times with methanol solution at 6500r for 10min, then disperse in isopropanol solution to obtain SiO 2 AuNR@SiO with a layer thickness of 7nm 2 solution, another 6ml solution was centrifugally dispersed in 6mL water to prepare AuNR@SiO 2 Solution A1 is ready for use.

[0054] 2) AuNR@SiO 2 @NH 2 Preparation of the solution: Add 10 mL of AuNR@SiO to 80 °C oil bath 2 Add 3 μL of APTES (3-aminopropyltriethoxysilane) solution to the solution, stir and react for 4 hours, wash the obtained product with methanol s...

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Abstract

The invention discloses a preparing method of a reinforced gold nanoparticle cluster fluorescence system based on surface plasma. The preparing method comprises the steps of conducting contact reaction of gold nanoparticle solution and modified gold nanorod solution with buffer solution to prepare the reinforced gold nanoparticle cluster fluorescence system based on surface plasma; among them, modified gold nanorod in the gold nanorod solution comprises gold nanorod, a silicon dioxide layer and an amine modified layer, wherein the silicon dioxide layer is covered outside the gold nanorod, and the amine modified layer is distributed on the outer surface of the silicon dioxide layer; the outer protecting group of the gold nanoparticle in the gold nanoparticle solution is chosen from one kind or multiple kinds of BSA (Bovine Serum Albumin), HAS (human serum albumin) and glutathione. The gold nanoparticle cluster fluorescence system prepared from the method has excellent fluorescence quantum yield.

Description

technical field [0001] The invention relates to gold nanoclusters, in particular to a method for preparing a gold nanocluster fluorescent system based on surface plasmon enhancement. Background technique [0002] Gold nanoclusters (AuNCs) are molecular aggregates composed of a few to dozens of atomic cores and organic single molecules such as thiol compounds or proteins as protective groups. AuNCs have become one of the current research hotspots due to their ideal optical and electrical properties. Studies have shown that when AuNCs are gradually reduced to the equivalent of the Fermi wavelength of electrons (usually <1.5nm), due to the two-word size effect, they will exhibit similar characteristics to semiconductors, generate discrete energy levels, and will excite fluorescence. [0003] Compared with traditional fluorescent markers, AuNCs have many advantages, such as: high biocompatibility, low toxicity, photostability, can be synthesized under very mild conditions, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/59C09K11/58C09K11/06
CPCC09K11/02C09K11/06C09K11/582C09K11/592C09K2211/10
Inventor 夏云生陈露宫素芹张慧朱慧刘春秀朱霜霜吕扬
Owner 天津市源创环保设备有限公司
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