A kind of fluorescent nanoparticle and its synthesis method, application

A technology of fluorescent nanoparticles and copolymers, applied in the field of fluorescent nanoparticles and their synthesis, to achieve the effects of good stability, weak scattering ability, and narrow emission peaks

Active Publication Date: 2019-09-27
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a fluorescent nanoparticle for temperature detection. The novel nanoprobe prepared by it has the characteristics of temperature sensitivity and luminescent property not affected by pH and ionic strength, which solves the problems existing in the existing intracellular temperature detection. question

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  • A kind of fluorescent nanoparticle and its synthesis method, application
  • A kind of fluorescent nanoparticle and its synthesis method, application
  • A kind of fluorescent nanoparticle and its synthesis method, application

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1, the preparation of fluorescent nanoparticles based on CdSeS quantum dots based on methacrylic acid-methyl methacrylate copolymer as matrix material

[0046] Get methacrylic acid-methyl methacrylate copolymer (the number-average molecular weight is 50,000, carboxyl group mass fraction: 1.0%) and CdSeS quantum dots are dissolved in tetrahydrofuran respectively, prepare the concentration of acrylic acid-methyl methacrylate copolymer Tetrahydrofuran solution of 10mg / mL, quantum dot concentration of 0.5mg / mL. Under stirring, the mixed solution containing 100 μL of the copolymer solution and 300 μL of the quantum dot solution was added dropwise into 5.0 mL of water, and the stirring was continued for 10 min. Evaporate tetrahydrofuran at 30° C., and centrifuge to obtain precipitates that are fluorescent nanoparticles; redisperse the obtained precipitates in pure water to obtain a sol of fluorescent nanoparticles containing carboxyl groups on the surface.

[0047...

Embodiment 2

[0049] Example 2, Preparation of fluorescent nanoparticles based on CdSeS quantum dots based on styrene-methacrylic acid block copolymer

[0050] The copolymer in Example 1 is replaced by styrene-methacrylic acid block copolymer (the number average molecular weight is 100,000, styrene group mass fraction: 40%, carboxyl group mass fraction: 25%), concentration is 10mg / mL, prepared fluorescent nanoparticles based on CdSeS quantum dots based on styrene-methacrylic acid block copolymer. Such as Figure 5 As shown, the dynamic light scattering test results show that the average particle size of the prepared fluorescent nanoparticles is 38nm, and the particle size distribution range is 35-46nm. Figure 6 It is the UV-visible absorption spectrum and photoluminescence spectrum of the prepared fluorescent nanoparticle sol. It can be seen from the figure that the fluorescent nanoparticle has strong absorption in the visible and ultraviolet regions, and its main absorption peak is loca...

Embodiment 3

[0051] Example 3, Preparation of fluorescent nanoparticles based on CdSe quantum dots based on methacrylic acid-methyl methacrylate copolymer

[0052] Dissolve methacrylic acid-methyl methacrylate copolymer (number-average molecular weight: 5,000, carboxyl group mass fraction: 10%) and CdSe quantum dots in dimethylformamide to prepare methacrylic acid-methacrylic acid A dimethylformamide solution with a methyl ester copolymer concentration of 10 mg / mL and a CdSe quantum dot concentration of 0.5 mg / mL. Under the condition of stirring, the mixed liquid containing 100 μL of copolymer solution and 100 μL of quantum dot solution was added dropwise into 5.0 mL of water, and the stirring was continued for 10 min. Dimethylformamide was removed by vacuum rotary evaporation at 30° C., centrifuged, and the resulting precipitate was redispersed in pure water to prepare a sol of fluorescent nanoparticles containing carboxyl groups on the surface.

[0053] Such as Figure 7 As shown, the ...

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Abstract

The invention relates to fluorescent nano-particles. The fluorescent nano-particles comprise quantum dots and high-molecular copolymers coating outside of the quantum dots. A novel nano-probe prepared from the fluorescent nano-particles has the characteristics of temperature sensitivity and independent luminance from pH and ion strength, and the existing problem for detecting the temperature in cells can be solved.

Description

technical field [0001] The invention relates to a fluorescent nanoparticle used for temperature detection, a synthesis method and an application thereof. Background technique [0002] Temperature is a very important physical parameter. Accurate and sensitive temperature detection is crucial for scientific research and industrial production. For example, when a person is sick and has a fever, it is often accompanied by an increase in body temperature; the temperature of tumor cells is often higher than that of normal cells. Therefore, detecting the temperature of cells not only helps people to further understand the metabolism of cells, but also has important clinical application value for disease prevention and diagnosis. [0003] Cells are mostly in the micron scale, and traditional thermometers such as thermocouples, thermal resistances, and radiation thermometers are difficult to use for temperature detection of cells. In order to measure cell temperature at the micron ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/88A61B5/00
Inventor 韩荣成降雨强沙印林
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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