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Polypeptide with function of reducing uric acid and application of polypeptide

A technology for reducing uric acid and activity, applied in the field of peptides

Active Publication Date: 2017-01-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the use of tripeptides to reduce uric acid levels in vitro or in vivo has not been reported in the literature

Method used

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  • Polypeptide with function of reducing uric acid and application of polypeptide
  • Polypeptide with function of reducing uric acid and application of polypeptide
  • Polypeptide with function of reducing uric acid and application of polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Take 100μl 18mg / ml Tyr-Glu-Gly solution in a 10ml centrifuge tube, add 50μl xanthine oxidase, incubate in a water bath at 30°C for 10min; add 2.9ml of the Tris-HCl buffer solution with pH 8.0, 400μl xanthine Standard stock solution, kept at 30°C for 20 minutes in a water bath; after the reaction, add 150 μl of 1M HCl, pass through a 0.22 μm aqueous membrane after cooling, replace the peptide sample with pH 8.0 Tris-HCl buffer as a blank control, and wait for the test.

Embodiment 2

[0042] Take 100μl 16mg / ml Tyr-Glu-Gly solution in a 10ml centrifuge tube, add 50μl xanthine oxidase, incubate in a water bath at 30°C for 10min; add 2.9ml of the Tris-HCl buffer solution with pH8. The stock solution was incubated in a water bath at 30°C for 20 minutes; after the reaction, 150 μl of 1M HCl was added, cooled and passed through a 0.22 μm aqueous membrane, and the peptide sample was replaced with pH 8.0 Tris-HCl buffer as a blank control, to be tested.

Embodiment 3

[0044] Take 100μl 14mg / ml Tyr-Glu-Gly solution in a 10ml centrifuge tube, add 50μl xanthine oxidase, incubate in a water bath at 30°C for 10min; add 2.9ml of the Tris-HCl buffer solution with pH8. The stock solution was incubated in a water bath at 30°C for 20 minutes; after the reaction, 150 μl of 1M HCl was added, cooled and passed through a 0.22 μm aqueous membrane, and the peptide sample was replaced with pH 8.0 Tris-HCl buffer as a blank control, to be tested.

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Abstract

The invention relates to a polypeptide with a function of reducing uric acid and an application of the polypeptide. The polypeptide with the function of reducing uric acid has an amino acid sequence as follows: Tyr-Glu-Gly. The polypeptide is synthesized with a solid-phase synthesis method by the use of a polypeptide synthesizer. In-vitro uric acid reduction activity detection is performed on the polypeptide with an HPLC method, and a result proves that the polypeptide has a good inhibition effect on xanthine oxidase and has excellent uric acid reduction activity. The polypeptide can be used as an adjuvant therapy drug for the gout disease or used in functional health-care food or medicines with the function of reducing uric acid.

Description

technical field [0001] The invention relates to the technical field of polypeptides, in particular to a polypeptide with uric acid-lowering activity and applications thereof. Background technique [0002] In recent years, with the improvement of people's dietary structure, the prevalence of gout at home and abroad has increased year by year, and there is a trend of younger people. Hyperuricemia is the biochemical basis of gout. It mainly refers to a disease caused by long-term purine metabolism disorder, resulting in excessive production of uric acid or insufficient excretion of uric acid. More and more studies have shown that hyperuricemia is not simply a disease of excessive uric acid content, it is closely related to a variety of cardiovascular and cerebrovascular diseases and kidney diseases, in addition, it is also associated with type Ⅱ diabetes and insulin resistance Signs are also inextricably linked. [0003] At present, the treatment of hyperuricemia mainly inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/087A61K38/06A61P19/06A23L33/18
CPCA23V2002/00A61K38/00C07K5/0812A23V2200/30A23V2250/55
Inventor 任娇艳康小燕
Owner SOUTH CHINA UNIV OF TECH
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