Embryo rescue method for overcoming cross-incompatibility of upland cotton-turneri cotton amphidiploid

Abstract

The invention discloses an embryo rescue method for overcoming cross-incompatibility of upland cotton-turneri cotton amphidiploid, comprising the steps of 1) hybridizing upland cotton with upland cotton-turneri cotton amphidiploid; 2) drip-applying gibberellin: drip-applying gibberellin aqueous solution to bracts on the pollination day; 3) culturing ovules; 4) grating seedlings: grafting the seedlings cultured in step 3) to Gossypium barbadense rootstocks. The method of the invention is efficient in preventing early dropping of bolls and avoiding pre-mortality of embryos, hybrids of upland cotton-turneri cotton amphidiploid with upland cotton are acquired successfully, upland cotton-turneri cotton pentaploid (sesquidiploid) material is acquired, fertility of such material is greatly improved as compared with the amphidiploid, the pentaploid is back-crossed successfully with upland cotton parents to obtain back-crossed offspring groups, and basis is laid for breeding utilization of the cotton species.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to an embryo rescue method for overcoming the hybrid incompatibility between upland cotton-Turneria amphidiploid and upland cotton. Background technique [0002] Cotton (Gossypium spp.) is the most important economic crop and textile raw material in the world. China is not only a big cotton producer, but also a big consumer. Among them, upland cotton is the most widely planted area, accounting for more than 90% of the total cotton planting area. However, the genetic basis of upland cotton varieties is very narrow, and it is difficult to achieve significant progress in breeding by using traditional breeding methods for interbreeding. [0003] Cotton Turner has excellent characteristics such as early shedding of bracts, no debris in lint, insect resistance, and salt tolerance. If the early shedding of bracts can be transferred to cultivated cotton, it will be conven...

AI Technical Summary

Problems solved by technology

However, artificial amphidiploids are highly sterile in both sexes

Nanjing Agricultural University used it as the male parent and the female parent respectively, and adopted the conventional hybridization method to carry out a large number of hybridizations with upland cotton for many years, but no real hybrid offspring was obtained. The hybrid incompatibility between the amphidiploid and upland cotton has become The biggest obstacle to the transfer of Turner cotton's excellent genes to upland cotton is that during the development of hybrid embryos, due to hybrid incompatibility, young embryos die within 3-5 days, resulting in the inability to obtain hybrid offspring