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Method for producing gamma-polyglutamic acid by using beer lees fermentation

A technology of polyglutamic acid and brewer's grains, applied in the field of bioengineering, can solve the problem of high cost of fermentation medium and achieve the effect of low processing cost

Inactive Publication Date: 2017-01-04
SHANDONG JIANZHU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for producing gamma-polyglutamic acid by fermenting brewer's grains, which solves the current problems of brewer's grains polluting the environment and the high cost of gamma-polyglutamic acid fermentation medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] γ-Polyglutamic acid was prepared by the following method:

[0023] 1. Dry the wet brewer's grains in an oven at 105°C until the water content is about 10%, then mechanically pulverize and sieve through a 20-40 mesh sieve.

[0024] 2. Add 1 mol / L nitric acid solution until the brewer's grains are completely submerged, and hydrolyze at 60°C for 3 hours.

[0025] 3. After the hydrolyzate was cooled to normal temperature naturally, 10mol / L sodium hydroxide solution was added to adjust the pH to 7.0, and the hydrolyzate was obtained after centrifugation at 4000r / min.

[0026] 4. Add 4% sodium glutamate to each 1000mL of the hydrolyzate to prepare a fermentation medium, and sterilize at 121°C for 15 minutes before use.

[0027] 5. Inoculate with 3% Bacillus subtilis Z-115 and ferment at 37℃ for 50h.

[0028] 6. The fermentation broth was centrifuged at 4000r / min for 10min to remove the bacteria, the supernatant was added with 2.5 times the volume of absolute ethanol, shaken...

Embodiment 2

[0030] The method of Example 1 was used to prepare γ-polyglutamic acid. The difference was that the process used in Step 2 was: adding 1 mol / L nitric acid solution until the brewer's grains were completely submerged, and hydrolyzing at 70°C for 3 hours. The final yield of γ-polyglutamic acid was 18.8 g / L.

Embodiment 3

[0032] The method of Example 1 is used to prepare γ-polyglutamic acid. The difference is that the process used in step 5 is: inoculate 5% Bacillus subtilis, ferment at 37 ° C for 60 hours, and the final γ-polyglutamic acid The yield was 19.2 g / L.

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Abstract

The invention provides a method for producing gamma-polyglutamic acid by using beer lees fermentation. The method comprises: drying beer lees, crushing, adding nitric acid, hydrolyzing, adding alkali to neutralize, preparing a fermentation culture medium, adding Bacillus subtilis, fermenting, carrying out solid-liquid separation, and then extracting the gamma-polyglutamic acid from the fermentation broth. According to the present invention, the process is simple, the operation is convenient, the environment pollution problem of the beer lees in the prior art is solved, and the high raw material cost problem of the gamma-polyglutamic acid production is solved.

Description

technical field [0001] The invention relates to a method for producing γ-polyglutamic acid by utilizing brewer's grains fermentation, and belongs to the field of biological engineering. Background technique [0002] Brewer's grains are the main by-product of the beer industry, which is the residue after the extraction of soluble carbohydrates in the seeds from barley through fermentation. For every 100kg of raw materials put into production, a beer enterprise will produce 120-130kg of wet brewer's grains (50%-75% moisture content). Because of its high moisture content, unsuitable for long-term storage, perishable, and inconvenient for transportation, most manufacturers in my country directly sell it to farmers at low prices as feed for poultry and pigs, cattle, sheep and other livestock. However, as feed The amount of use is limited, so a few manufacturers even directly discharge it as waste, wasting resources and seriously damaging the ecological environment near the brewer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C12R1/125
Inventor 张超薛维纳陈文兵李洪李琳琳侯书国赵海涛
Owner SHANDONG JIANZHU UNIV
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