Method for acquiring broccoli microspore material in winter

Abstract

The invention discloses a method for acquiring a broccoli microspore material in winter. The method comprises the following steps: a, sowing seeds, namely, sowing seeds of broccoli into a shallow layer of a hole tray; b, accelerating germination, namely, putting the hole tray on a hotbed for accelerating germination at 20-25 DEG C at daytime and 10-15 DEG C at night; c, performing fixed planting, namely, when 2-3 true leaves grow out, performing fixed planting and cultivation in medium nutrient cups, and irrigating with a nutrient solution MB1 at regular time; d, performing low-temperature vernalization, namely, after 14-16 true leaves grow out, performing low-temperature vernalization for about 20 days at 8-15 DEG C, and irrigating with a nutrient solution MB2; e, cultivating, namely, after ball-flowers are formed, irrigating with a nutrient solution MB3, cultivating continuously, and controlling the ambient temperature inside a greenhouse to be 20-25 DEG C so as to obtain normal flower buds; f, extracting microspores, namely, sterilizing selected flower buds, and extracting pollen, thereby obtaining the microspore material. By adoption of the soilless cultivation technique, the flower buds applicable to extraction of the microspore material can be cultivated in the greenhouse in winter, and the technical difficulty that broccoli microspores are hard to obtain normally in winter can be solved.

Description

technical field [0001] The invention relates to a method for obtaining microspore materials, more particularly to a method for obtaining broccoli microspore materials in winter. Background technique [0002] Broccoli, also known as broccoli, green cauliflower, stem broccoli, etc., is a primary or secondary herbaceous plant in the Brassicaceae Brassica species with green curds as the product organ. As a product, the curd is rich in nutrition, good in color, aroma and taste, and is a famous and special vegetable that is very popular in the international market. [0003] Since Lichter reported for the first time in 1982 that rapeseed free microspore culture obtained regenerated plants, Brassica crops, especially the microspore culture of rapeseed, have achieved remarkable results in terms of embryo emergence rate, embryo yield, embryo culture and regenerated plants. Great success. Compared with Brassica napus, head cabbage and other crops, the cultivation of free microspores ...

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Problems solved by technology

[0004] Moreover, in the normal flowering season of broccoli, a large amount of microspores can be obtained; but when the suitable flowering season is over, after entering autumn and winter, due to the unfavorable external cultivation conditions, flower buds suitable for microspores cannot be normally drawn out, and then cannot be produced in the broccoli. Obtain broccoli microspores in winter to provide resources for creating DH (double haploid) materials, which brings more difficulties to broccoli microspore cultivation and breeding technology

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