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Saussurea-obvallata effective part and preparing method and application thereof

A technology of effective parts and parts, applied in the field of plant extracts, can solve problems that have not been seen yet, and achieve good anti-tumor effect

Inactive Publication Date: 2016-12-21
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research and application of Saussurea bractosa on the activity of inhibiting HDAC1 enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiments 1 to 3 are specific implementations of the effective parts of Snow Lotus bractosa and its preparation method and application of the present invention, wherein Embodiment 1 is the best embodiment.

[0023] Example 1

[0024] The preparation method comprises the following steps: pulverizing the whole snow lotus herb, ultrasonically extracting it with an ethanol solution with a mass percentage concentration of 75% to 85% for 1 hour, then filtering, and adding the filter residue to reflux extraction with a mass percentage concentration of 75% to 85% ethanol solution to obtain Reflux the extract twice for 0.5 hours each time, combine the extracts, concentrate and dry to obtain the extract; add distilled water to disperse the extract to obtain the extract dispersion, and then sequentially use petroleum ether, ethyl acetate and n-butanol as solvents for extraction The extract dispersion liquid, after the solvent is evaporated, the petroleum ether part, the ethyl ac...

Embodiment 2

[0026] The preparation method comprises the following steps: pulverizing the whole snow lotus herb, ultrasonically extracting it with an ethanol solution with a mass percentage concentration of 85% to 95% for 3 hours, then filtering, and adding the filter residue to reflux extraction with an ethanol solution with a mass percentage concentration of 85% to 95%. Once, each time for 1 hour, the extracts were combined, concentrated and dried to obtain an extract; the extract was dispersed with distilled water to obtain an extract dispersion, and then petroleum ether, ethyl acetate and n-butanol were used as solvents to extract the extract dispersion, After evaporating the solvent, the petroleum ether fraction, the ethyl acetate fraction and the n-butanol fraction of Saussurea brachyphylla are obtained; the extract dispersion remaining after extraction is concentrated and dried to obtain the water-phase extracted fraction of Saussurea brachiae.

Embodiment 3

[0028] The preparation method comprises the following steps: crushing the whole snow lotus herb, ultrasonically extracting it with an ethanol solution with a mass percentage concentration of 65% to 75% for 0.5 hours, then filtering, adding an ethanol solution with a mass percentage concentration of 85% to 95% to the filter residue and refluxing extraction 2 times, 2 hours each time, combine the extracts, concentrate and dry to obtain the extract; add distilled water to disperse the extract to obtain the extract dispersion, and then sequentially use petroleum ether, ethyl acetate and n-butanol as solvents to extract the extract dispersion After the solvent is evaporated, the petroleum ether part, the ethyl acetate part and the n-butanol part of Saussurea bractosa are obtained; the remaining extract dispersion after extraction is concentrated and dried to obtain the aqueous phase extraction part of Saussurea bractosa .

[0029] Performance Testing

[0030] Take 0.4 mg of dried ...

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PUM

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Abstract

The invention discloses a saussurea-obvallata effective part and a preparing method and application thereof, and belongs to the technical field of plant extract. The saussurea-obvallata effective part is selected from the mixture of any mass ratio of one or two above of a saussurea-obvallata petroleum ether part, a saussurea-obvallata ethyl acetate part, a saussurea-obvallata n-butyl alcohol part and a saussurea-obvallata aqueous phase extraction part. The preparing method includes the steps that saussurea-obvallata whole plant is smashed, an ethanol solution with the mass percent concentration of 65% to 95% is added and subjected to reflux extraction, the obtained extracted liquid is concentrated and dried, and extract is obtained; the extract is dispersed in distilled water, extract dispersion liquid is obtained, petroleum ether, ethyl acetate and n-butyl alcohol are sequentially used as a solvent to extract the extract dispersion liquid, and after the solvent is volatilized, the saussurea-obvallata petroleum ether part, the saussurea-obvallata ethyl acetate part and the saussurea-obvallata n-butyl alcohol part are obtained; the extracted extract dispersion liquid is concentrated and dried, and the saussurea-obvallata aqueous phase extraction part is obtained. The saussurea-obvallata effective part has an application to preparing medicine for inhibiting a HDAC1 enzyme, and can be used for preparing antitumor medicine.

Description

technical field [0001] The invention relates to the effective part of Saussurea bractosa and its preparation method and application, and belongs to the technical field of plant extracts. Background technique [0002] Saussurea obvallata (DC.) Edgew. is a plant of the subgenus Saussurea genus Saussurea, also known as "Bacteria obvallata", a perennial herb with a height of 16-60 cm [see: Zhang Zhiyun. Flora of China. Vol. 78 [M]. Beijing: Science Press, 1999: 27.]. Its flowers are blue-purple, and the achenes are oblong and 5 mm long. The flower and fruit period is from July to September. It is born in alpine grasslands, flowstone beaches, crevices by streams, and rocky hillsides, at an altitude of 3200-4700 meters. Saussurea braculata is a unique plant in the plateau area, which has unique pharmacological activity. It is warm in nature and slightly bitter. Generally, the whole herb is used as medicine. It is mainly used to treat rheumatoid arthritis, blood deficiency, infer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P35/00
CPCA61K36/28A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 芦永昌吴疆李海丽朱奎德钱帅白雪王欢田静
Owner QINGHAI UNIV FOR NATITIES
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