Preparation method of microscope lens oil capable of transparentizing microporous filter membrane

A technology of transparent microporous filter membrane and microscope lens oil, which is applied in microscopes, optics, instruments, etc., can solve the problems of microscopic observation and opacity without a good oil lens, so as to facilitate detection and scientific research experiments, clear objects, The effect of increased light

Active Publication Date: 2016-12-14
湖南天骑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The prior art adopts the transparent method of the microporous membrane to refer to the formation of a transparent carrier to facilitate observation under a microscope, but the carrier formed in this case cannot directly use the microscopic method of the oil lens, cedar oil will affect the microporous membrane. The added substances make the originally transparent microporous membrane opaque. Therefore, there is currently no good way to use the microporous membrane as a carrier for oil microscope observation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: The present invention is used for the detection experiment of Mycobacterium tuberculosis:

[0025] The experimental process includes: making the sample to be tested → → using the membrane separation device to concentrate the sample, the Mycobacterium in the sample to be tested adheres to the filter membrane → → staining and cleaning the bacteria on the filter membrane → → adding to the membrane separation device alcohol, and other liquids in the filter membrane pores are excluded. At this time, there is only alcohol in the filter membrane pores → → the alcohol in the filter membrane pores is volatilized, the filter membrane pores are filled with air, and the filter membrane at this time is opaque → →Take out the filter membrane from the membrane separation device, add microscope oil prepared according to the present invention to the filter membrane, which can make transparent microporous filter membrane, the filter membrane hole is filled with membrane tran...

Embodiment 2

[0027] Embodiment 2: The present invention is used for cell detection experiment:

[0028] The experimental process includes: making the sample to be tested → → using the membrane separation device to concentrate the sample, and the cells in the sample to be tested are attached to the filter membrane → → stain and wash the cells on the filter membrane → → remove the filter membrane from the membrane separation device , add the microscope oil prepared according to the present invention to the filter membrane, which can make transparent microporous filter membrane, the filter membrane hole is filled with membrane transparent liquid, and the filter membrane is transparent → → move the sample to the bottom of the oil lens, drop 1-2 drops When the mirror oil is on the object to be measured, because the refractive index of the mirror oil and the lens is close or equal, the light transmittance is high, the magnification is high, and the object is clear → → observation and analysis.

...

Embodiment 3

[0030] Embodiment 3: used for air quality detection experiment:

[0031] The experimental process includes: air sampling→→use the membrane separation device to concentrate the sample, the suspended matter in the air sample adheres to the filter membrane, and the filter membrane is opaque at this time→→take out the filter membrane from the membrane separation device, and put it into the filter membrane. Add the microscope oil prepared according to the present invention that can transparent microporous filter membrane, the filter membrane hole is filled with membrane transparent liquid, the filter membrane is transparent →→ Move the sample to the bottom of the oil lens, drop 1-2 drops of lens oil to be tested On the object, because the refractive index of the mirror oil and the lens is close or equal, the light transmittance is high, the magnification is high, and the object is clear → → observe and count.

[0032] The composition of the microscope oil that can be transparent mi...

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PUM

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Abstract

The invention discloses a preparation method of microscope lens oil capable of transparentizing a microporous filter membrane. According to a refraction coefficient of a microscope lens, membrane transparent liquid with the refraction coefficient which is the same as or similar with the refraction coefficient of the microscope lens is selected as the lens oil; meanwhile, in a process of detecting components intercepted by the microporous filter membrane, the lens oil is added into the microporous filter membrane, so that the microporous filter membrane has a transparent state; a material of the microporous filter membrane has a transparent material property; the prepared microporous filter membrane is at a non-transparent or semi-transparent state; the error between an refraction index of the lens oil and the refraction index of the material of the microporous filter membrane is no more than +/-10 percent. The invention aims at providing the preparation method of the microscope lens oil capable of transparentizing the microporous filter membrane; one type of liquid is prepared and a non-transparent microporous filter membrane is changed into a completely transparent microporous filter membrane; the liquid is also used as the lens oil in an oil lens observation process and an unprecedented convenient and effective novel method is provided for morphological detection.

Description

technical field [0001] The invention relates to a liquid preparation method for simultaneously using a transparent microporous filter membrane and as microscope oil in the inspection and detection process using membrane separation technology in the fields of medicine, biology, environment and the like. Microscope oil preparation method for pore filter membrane. Background technique [0002] As we all know, the microscope is an optical instrument used to observe small objects. Usually, the microscope cannot be directly observed, because the microscope must be in a transparent background to observe the specific shape, usually using a glass slide. When the microscope requires a high magnification, the lens is very small. When the light passes through medium objects of different densities (glass → air → lens), part of the light will be refracted and lost, and the light entering the lens barrel is less, and the field of view is dark. , the object cannot be seen clearly. For exa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/33G02B21/34
CPCG02B21/33G02B21/34
Inventor 彭钧
Owner 湖南天骑生物科技有限公司
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