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Preparation of temperature sensitive quinine chiral sensor and application thereof in electrochemical recognition of tryptophan enantiomer

A tryptophan enantiomer and sensor technology, applied in the fields of biotechnology and electrochemical research, can solve the problems of complex operation, inability to detect, weak absorption, etc., and achieve the effects of simple preparation method and excellent identification performance.

Active Publication Date: 2016-12-14
CHANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional methods for detecting amino acids mainly include spectroscopic methods (including ultraviolet-visible spectroscopy, fluorescence, chemiluminescence, etc.), capillary electrophoresis, high-performance liquid chromatography, and nuclear magnetic resonance. However, most amino acids have extremely weak absorption in the ultraviolet-visible spectral region. There is no fluorescence, so it cannot be directly detected
These methods usually need to derivatize the sample to improve the sensitivity and selectivity of the detection, the operation is complicated and the cost is high

Method used

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  • Preparation of temperature sensitive quinine chiral sensor and application thereof in electrochemical recognition of tryptophan enantiomer
  • Preparation of temperature sensitive quinine chiral sensor and application thereof in electrochemical recognition of tryptophan enantiomer
  • Preparation of temperature sensitive quinine chiral sensor and application thereof in electrochemical recognition of tryptophan enantiomer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of quinine chiral sensor includes the following steps:

[0020] (1) The experiment adopts a three-electrode system, the glassy carbon electrode (diameter 3mm) is used as the working electrode, the platinum sheet electrode is used as the counter electrode, and the saturated calomel electrode (SCE) is used as the reference electrode, which is immersed in the prepared 5mmol / L quinine solution In , a quinine-modified electrode was prepared by applying a potential deposition of -1.0V for 600s by the potentiostatic method.

[0021] (2) The experiment adopts a three-electrode system. The quinine-modified electrode is used as the working electrode, the platinum electrode is used as the counter electrode, and the saturated calomel electrode (SCE) is used as the reference electrode. / D-tryptophan solution, after standing still for 60s, carry out differential pulse scanning, the recognition effect diagram of tryptophan enantiomer is shown in figure 1 , it can be s...

Embodiment 2

[0023] The preparation of the temperature-sensitive quinine chiral sensor and its application in electrochemical recognition of tryptophan enantiomers are the same as in Example 1.

[0024] At different temperatures, the effect of quinine chiral sensor on the recognition of tryptophan enantiomers was investigated. The prepared quinine-modified electrodes were respectively immersed in the prepared L- / D-tryptophan solution with a concentration of 0.5mmol / L. After standing for 60s, differential pulse scanning was performed. The experimental temperature was 0-40°C. The recognition effects of tryptophan enantiomers at different temperatures are shown in figure 2 , it can be seen that the chiral sensor has a better recognition effect on D-tryptophan at 8°C, and has a better recognition efficiency on L-tryptophan at 25°C.

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Abstract

The invention relates to a preparation of a temperature sensitive quinine chiral sensor and application thereof in electrochemical recognition of a tryptophan enantiomer. The invention includes the following steps: preparation of a quinine chiral sensor and electrochemical recognition of a tryptophan enantiomer. The invention has the beneficial effects that the preparation method of the quinine chiral sensor is simple, and uses the chiral environment character of quinine for preparation of a modified electrode with excellent system performance. At different temperatures, the chiral sensor has the opposite identification effect on an L- / D- tryptophan.

Description

technical field [0001] The invention relates to the preparation of a temperature-sensitive quinine chiral sensor and its application in electrochemical recognition of tryptophan enantiomers, belonging to the fields of biotechnology and electrochemical research. technical background [0002] Chirality is a basic property of nature, which expresses the characteristics of asymmetric molecular structure of compounds, and widely exists in the fields of life science, medicine, pharmaceutical science and food science. Especially in chiral drugs, because the drug properties are closely related to the three-dimensional structure of drug molecules, although enantiomers with different configurations have the same physical properties, there are differences in the metabolic process, drug efficacy and toxicity in vivo. In most cases, only one of the enantiomers of chiral drugs has pharmacological activity, while the other enantiomer has no activity and may even have the opposite pharmacol...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/308G01N27/48
Inventor 孔泳于茵欧洁
Owner CHANGZHOU UNIV
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