Laying duck feather picking character important gene HTR2C molecular marker and application

Abstract

The invention discloses a laying duck feather picking character important gene HTR2C molecular marker. The molecular marker comprises the following steps of 1, DNA extraction, wherein DNA is extracted from a laying duck pectoral muscle sample; 2, primer design, wherein Primer Primer 5.0 is applied for primer design; 3, PCR amplification, wherein a 20-microliter reaction system is applied, PCR amplification conditions are set for PCR amplification, and 5 microliters of a product is taken for detecting 2.0% agarose gel electrophoresis; 4, amplification product fragment purification, carrier connection, conversion and positive clone screening and sequencing; 5, SNP mutation site screening and association analysis, and determination of SNP related to feather picking characters. The method is applied for searching for the molecular marker related to feather picking characters so that feather picking caused by genetic factors can be eliminated. References can be provided for building the method for selecting molecules with behavior characters.

Description

technical field [0001] The invention belongs to a poultry molecular marker selection method, in particular to a molecular marker method for laying ducks. Background technique [0002] Behavior, as a complex quantitative trait, is regulated by multiple genes and affected by various environmental factors. Among these many behaviors, feather pecking behavior (non-nutritional deficiency) has always attracted people's attention, because it has become the main problem affecting animal welfare in large-scale intensive poultry farming in Europe, America and China. Feather and aggressive pecking. Aggressive pecking is defined as a rapid attack on the head, body and anus of the opponent. In severe cases, the poultry skin can be maimed or cannibalism. It has been reported abroad that the interleukin and serotonin receptor gene HTR2C in laying hens is related to feather pecking behavior and feather damage, but it has not been reported in laying ducks. [0003] Among the two authorized...

AI Technical Summary

Problems solved by technology

In recent years, there have been a large number of research reports on molecular markers and their application in breeding, but there are few reports on the mining and application of molecular markers related to behavioral traits, especially the research and application of molecular markers on feather pecking behavior of laying ducks is still a new field. field

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