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A method for efficiently transfecting turbot muscle cells

A technology of muscle cells and turbot, applied in the field of high-efficiency transfection of turbot muscle cells, can solve the problems of low transfection efficiency and restrict the development of research fields, and achieve high transfection efficiency, fast translation, and good cell growth status Effect

Active Publication Date: 2020-01-14
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the immature transfection method with extremely low transfection efficiency seriously restricts the further development of related research fields.

Method used

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  • A method for efficiently transfecting turbot muscle cells
  • A method for efficiently transfecting turbot muscle cells
  • A method for efficiently transfecting turbot muscle cells

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Experimental program
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Embodiment 1

[0035] 1) Recovery and culture of turbot muscle cell lines

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Abstract

The invention discloses a method for efficient transfection of turbot muscle cells. The transfection is realized by performing a nucleofection method on a constructed turbot muscle cell line serving as a material and a plasmid which is connected with a green fluorescent protein (GFP) gene which serves as an exogenous gene; the transfection efficiency is detected by a fluorescence inversion microscope and a flow cytometer. By the adoption of the transfection method disclosed by the invention, the transfection efficiency of the constructed turbot muscle cell line for the GFP gene can reach 65 percent, and cells have good growing states. The method is configured to supply a powerful tool to research the fields such as fish cell signal transduction, fish functional genomics, fish threpsology and aquatic environmental toxicology.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a method for efficiently transfecting turbot muscle cells. Background technique [0002] With the development of modern biotechnology, cell culture technology has become an important means of fish breeding, nutrient metabolism, immune regulation and other related research. Transient cell transfection technology, as an important means to study gene function, has been applied more and more widely. Especially with the deepening of relevant research level, cell transient transfection——gene overexpression technology (Overexpression) has become a necessary technical means for the current study of fish gene function and cell signal transduction. [0003] Conventional cell transfection methods include liposome transfection, electroporation transfection and virus infection transfection. At present, the liposome method and electroporation method are commonly used in gene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor 周慧慧王旋何艮麦康森高宗宇江浩文
Owner OCEAN UNIV OF CHINA
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