Disease-resistant microbial soil remediation agent and preparation method thereof
A technology of soil remediation agent and microbial agent, which is applied in the field of disease-resistant microbial soil remediation agent and its preparation, which can solve problems such as damage, low crop quality, and great environmental threats, meet the low requirements for production conditions, and improve soil structure and quality, the production process is convenient and simple
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[0017] The preparation method of above-mentioned anti-disease microbial soil restoration agent comprises:
[0018] The carrier was pulverized and sieved to obtain the first sample. Before the carrier is pulverized, a soil conditioner is preferably added therein. The soil conditioner is preferably chemical substances such as maleic acid, polyacrylic acid, and polyvinyl alcohol. The soil conditioner can increase the gap between the carrier molecules. In the subsequent steps, the bacterial agent can be more fully adsorbed; after crushing, sieve, preferably with a sieve hole of 0.1cm to 2cm, the carrier within this range has a good adsorption effect, and the sample particles will not be too small and inconvenient Next step.
[0019] Fermenting the microbial agent to obtain a second sample. When fermenting the microbial inoculum, the preferred method is to separately ferment different strains in the microbial inoculum to obtain a plurality of bacterial liquids, and then inoculate...
Embodiment 1
[0026] 100 kg of dried bentonite was weighed, 0.5 kg of maleic acid was added thereto and crushed, and passed through a sieve with a diameter of 0.5 cm to obtain a first sample.
[0027] Get Bacillus megaterium, jelly-like Bacillus, Paecilomyces lilacinus, Bacillus sporogenes and Bacillus subtilis each 10kg, in five petri dishes, the above five strains are respectively fermented, the present embodiment selects beef extract Agar medium; the temperature of the entire fermentation process is controlled at 33°C, and the existing intelligent temperature controller is selected to regulate and control the temperature of the entire fermentation process; at the beginning of the fermentation process, the pH of the fermentation environment is adjusted to 5.5 with citric acid. No further intervention or adjustment of the pH was carried out during the fermentation process. The whole fermentation process lasts continuously for 72 hours, and the bacterial liquid of each bacterial species is ...
Embodiment 2
[0031] Weigh 50kg of diatomaceous earth, 25kg of chicken manure and 25kg of duck manure and mix them, then add 0.5kg of polyacrylic acid and crush them, pass through a sieve with a diameter of 0.8cm, and obtain the first sample.
[0032] Get Bacillus megaterium, jelly-like Bacillus, Paecilomyces lilacinus, Bacillus lateralis and Bacillus subtilis, each 10kg of Bacillus licheniformis, in six petri dishes, above six bacterial classifications are fermented respectively, this implementation For example, pea agar medium is selected as the culture substrate; the temperature of the entire fermentation process is controlled at 36°C, and the existing intelligent temperature controller is selected to regulate and control the temperature of the entire fermentation process; at the beginning of the fermentation process, malic acid is used to adjust the pH of the fermentation environment Adjusted to 6, no pH intervention or adjustments were made during the remainder of the fermentation. The...
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