Adaptive de-noising method for Fourier ptychographic microscopy

Abstract

The invention discloses an adaptive de-noising method for Fourier ptychographic microscopy, and the method comprises the steps: taking an image under the condition that all light sources are turned off, and enabling the image to serve as a background light image Ib; sequentially turning on all LED units and taking a group of illuminating light images (shown in the description), enabling all illuminating light images to be subtracted by the background light image Ib, and obtaining a group of no-background-light images (shown in the description); carrying out the de-noising operation of all dark field images of the no-background-light images (shown in the description) according to a threshold value Tk, and gradually increasing the threshold value Tk from zero till the number of non-zero pixels in the dark field images is less than the half of the total number of pixels of the images; finally obtaining a group of dark field no-noise images (shown in the description), and calculating the ratio G of the mean values of the images (shown in the description), generated through an initial solution, and the dark field no-noise images in an iterative process of the Fourier ptychographic microscopy; employing the dark field no-noise images for updating if G is greater than 0.5 and less than 2, or else, carrying out no updating of the spectrums corresponding to the dark field no-noise images. The method improves the quality of the image reconstructed through the Fourier ptychographic microscopy, and stably and precisely constructs a large-view-field high-resolution image.

Description

technical field [0001] The invention belongs to the field of denoising of microscopic imaging technology, in particular to an adaptive denoising method aiming at Fourier stacked microscopic imaging technology. Background technique [0002] In the field of microscopic imaging, higher resolution has always been the goal of pursuit, but there is a key problem while improving the resolution, that is, the space-bandwidth product of the microscope does not increase with the resolution, in other words, the traditional microscope There is a contradiction between the resolution and the size of the field of view that is difficult to balance at the same time. Because traditional microscopes use low-magnification objective lenses for imaging, the field of view is large but the resolution is low, and when high-magnification objective lenses are used for imaging, the resolution is improved but the corresponding field of view will be reduced. At present, in order to break through the cont...

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Problems solved by technology

[0004] Fourier stack microscopic imaging technology is a new type of large-field high-resolution microscopic imaging technology, but its reconstruction quality is often affected by the noise in the captured low-resolution images, especially those with extremely small signals. , Dark-field images that are easily affected by noise will seriously affect the high-frequency information of the reconstructed image. Therefore, how to effectively remove the noise in the captured image so that it does not affect the final reconstruction quality has become a Fourier stack display. A technical problem that must be overcome in micro-imaging technology

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