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Frequency shift light field microscope and three-dimensional super-resolution microscopic display method

A light field microscope and frequency shifting technology, applied in the field of microscopy, can solve the problems of incompatibility and achieve the effect of improving imaging depth and resolution

Inactive Publication Date: 2016-12-07
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the above-mentioned microscopy techniques are often incompatible in both 3D microscopic display and super-resolution display.

Method used

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  • Frequency shift light field microscope and three-dimensional super-resolution microscopic display method
  • Frequency shift light field microscope and three-dimensional super-resolution microscopic display method
  • Frequency shift light field microscope and three-dimensional super-resolution microscopic display method

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Embodiment Construction

[0030] The present invention will be further described below with reference to the accompanying drawings and embodiments, but the protection scope of the present invention should not be limited by this.

[0031] The frequency-shifted light field microscope of this embodiment includes an illumination module, a microscope module, an optical path connection coupling module, and a light field acquisition module. The microscope microlens array is arranged in a honeycomb shape, and each microlens has a corresponding position behind each microlens. CCD, the illumination module of this microscope uses LED matrix illumination, and the LED lights are arranged in a rectangular shape.

[0032] like figure 1 As shown, the frequency-shifted light field microscope of this embodiment includes: a computer 1 as an image processing module, a CCD 2, a microlens array 3, a controllable shutter 4, a field lens 5, an objective lens 6, a stage 7 and an LED matrix 8 as illumination Module, behind eac...

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Abstract

The invention discloses a frequency shift light field microscope, and the microscope comprises an objective table, an illuminating module, a microscopic module, an image obtaining module, and an image processing module. The illuminating module is arranged below the objective table, and can irradiate a sample at different angles. The image obtaining module obtains the light field images which are formed by the irradiation of the sample from different angles. The image processing module obtains all depth images through the refocusing of the light field images, carries out the frequency spectrum iteration after all images at each depth are obtained, carries out the filtering of the obtained high-resolution images, and then carries out the three-dimensional reconstruction through the high-resolution images at different depths. The invention also discloses a three-dimensional super-resolution microscopic display method. The microscope and the display method combine the technology of frequency spectrum iteration and the technology of light fields together, improve the imaging depth and resolution, and can recover a better microscopic three-dimensional image with the depth.

Description

technical field [0001] The invention relates to the field of microscopy, in particular to a frequency-shifted light field microscope and a three-dimensional super-resolution microscopic display method. Background technique [0002] With the continuous development of microscopy technology, the way people obtain information on the microscopic world is more diversified, and the amount of information obtained is also increasing. Among several perception modes, optical perception is one of the most important channels for people to obtain information, which can obtain biological information without destroying cell activity. Conventional optical microscopy can only obtain two-dimensional images that do not exceed the optical diffraction limit. [0003] In order to solve the above problems, some researchers have proposed some microscopic three-dimensional display technologies. For example, the patent document with publication number CN1664558A discloses a miniature three-dimensiona...

Claims

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Application Information

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IPC IPC(8): G02B21/06G02B21/36G01B11/24
CPCG01B11/24G02B21/06G02B21/367
Inventor 李明李屹成温睿昕王子昂孙试翼匡翠方李海峰刘旭赵伟
Owner ZHEJIANG UNIV
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