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Tea polyphenol tea polysaccharide composition with synergistic, attenuating and anti-liver cancer effects

A tea polyphenol tea, anti-liver cancer technology, applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve the problem of lack of biological activity of tea polyphenols, insignificant curative effect, Low water solubility and other problems, to achieve the effect of improving bioavailability, improving quality of life, and improving solubility

Active Publication Date: 2020-02-21
清枫链食苏打饮品(吉林)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, tea polyphenols have low water solubility, are easily catabolized by the upper digestive tract and liver, and are quickly excreted through the urinary system, which prevents the biological activity of tea polyphenols from being maximized.
In addition, although tea polyphenols have certain anticancer activity, due to the characteristics of small molecules, they will indiscriminately penetrate all types of cells, thus bringing certain toxic and side effects
[0004] Tea polysaccharides play a powerful immune regulation function in cancer treatment, but the effect of single and short-term administration is not significant

Method used

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  • Tea polyphenol tea polysaccharide composition with synergistic, attenuating and anti-liver cancer effects
  • Tea polyphenol tea polysaccharide composition with synergistic, attenuating and anti-liver cancer effects
  • Tea polyphenol tea polysaccharide composition with synergistic, attenuating and anti-liver cancer effects

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Embodiment 1

[0026] A method for preparing a tea polyphenol tea polysaccharide composition with synergistic, attenuating and anti-liver cancer effects, comprising the following steps:

[0027] (1) Preparation of tea polyphenols: take tea leaves, pulverize them, add 15 times by mass of ethanol aqueous solution with a volume concentration of 80%; let stand for 48 hours, filter under reduced pressure to obtain filtrate and tea dregs; use 3 times of ethyl acetate for the filtrate The ester was extracted 5 times, allowed to stand for stratification, and the upper layers were combined, and the combined solution was concentrated to dryness under reduced pressure to obtain crude tea polyphenols; the crude tea polyphenols were dissolved in ethanol, and added to the treated AB-8 macroporous adsorption resin On the column, the ratio of diameter to height of the resin column is 1:12, firstly eluted with water until the eluent is colorless, then eluted with 4 column volumes of NaOH aqueous solution with...

Embodiment 2

[0031] A method for preparing a tea polyphenol tea polysaccharide composition with synergistic, attenuating and anti-liver cancer effects, comprising the following steps:

[0032] (1) Preparation of tea polyphenols: take tea leaves, pulverize them, add 10 times by mass of ethanol aqueous solution with a volume concentration of 75%; let stand for 72 hours, filter under reduced pressure to obtain filtrate and tea dregs; The ester was extracted 7 times, allowed to stand for stratification, and the upper layers were combined, and the combined liquid was concentrated to dryness under reduced pressure to obtain crude tea polyphenols; the crude tea polyphenols were dissolved in ethanol, and added to the treated D-101 macroporous adsorption resin On the column, the ratio of diameter to height of the resin column is 1:10, first eluted with water until the eluent is colorless, then eluted with 3 column volumes of 0.2% NaOH aqueous solution to remove the pigment, and finally with 10 The ...

Embodiment 3

[0036] A method for preparing a tea polyphenol tea polysaccharide composition with synergistic, attenuating and anti-liver cancer effects, comprising the following steps:

[0037] (1) Preparation of tea polyphenols: take tea leaves, pulverize them, add 20 times by mass of ethanol aqueous solution with a volume concentration of 99.7%; let stand for 24 hours, filter under reduced pressure to obtain filtrate and tea dregs; use 3 times of ethyl acetate for the filtrate Ester extraction 3 times, standing for stratification, combining the upper layers, concentrating the combined solution to dryness under reduced pressure to obtain crude tea polyphenols; On the column, the ratio of diameter to height of the resin column is 1:15, firstly eluted with water until the eluent is colorless, then eluted with 5 column volumes of NaOH aqueous solution with a mass concentration of 0.4% to remove the pigment, and finally with 12 The volume concentration of the column volume is 90% ethanol aqueo...

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Abstract

The invention discloses a tea polyphenol and tea polysaccharide composition capable of enhancing effects, reducing toxicity and resisting liver cancer. The composition comprises 0.5 to 2 parts of tea polyphenol and 1 part of tea polysaccharide, wherein the purity of the tea polyphenol is equal to or higher than 90%, and the molecular weight of the tea polysaccharide is 80 to 100kDa. The composition enhances the targeting of the tea polyphenol, reduces the toxicity, improves the solubleness of the tea polyphenol in a body fluid and improves the bioavailability of the tea polyphenol, the immunization of the tea polysaccharide is combined while the antioxidant activity of the tea polyphenol is given into full play and a free radical in a human body is cleared, and the tea polyphenol and the tea polysaccharide work together to achieve a remarkable effect of resisting the liver cancer. After the composition is taken, the side or toxic effects are reduced, the tea polyphenol and the tea polysaccharide are from one same material, so that the use ratio of the material is improved, and an adopted solvent can be recycled. In addition, the tea polysaccharide with the molecular weight of 80 to 100kDa, acquired through an ultrafiltration technique, has the characteristics of high efficiency, low cost, short period of production, mild conditions and the like.

Description

technical field [0001] The invention relates to a tea polyphenol and tea polysaccharide composition with synergistic, attenuating and anti-liver cancer effects and a preparation method, belonging to the technical application field of medicine and health care products. Background technique [0002] Liver cancer is a common type of cancer with high morbidity and mortality. Surgical treatment and radiotherapy and chemotherapy are the main means of treating liver cancer at present. However, surgical treatment has a poor prognosis and is prone to relapse; chemical drugs will bring great toxic and side effects because of their killing effect on normal cells, which will bring great pain to patients. Therefore, scientists are working hard to find more efficient and low-toxic anticancer agents from natural products. Studies have shown that the improvement of antioxidant levels and immune levels are two key means of tumor treatment. Free radicals will attack organs or tissues in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/82A61K47/36A61P35/00A61K31/715
CPCA61K31/715A61K36/82A61K47/36A61K2236/333A61K2236/55A61K2300/00
Inventor 陈海霞王京雅刘玮陈忠琴
Owner 清枫链食苏打饮品(吉林)有限公司
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