The transfer method of exogenous dna of Bacillus marine atrophaeus

A technology of Bacillus atrophicus, marine, applied in the field of microorganisms

Inactive Publication Date: 2019-11-01
SHANGHAI JIAOTONG UNIV
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  • Abstract
  • Description
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Problems solved by technology

So far, there have been no reports of Bacillus atrophaeus var. globigii, ahistorical biowarfare simulant. PLoS one. 2011,6:e17836.)

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  • The transfer method of exogenous dna of Bacillus marine atrophaeus
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  • The transfer method of exogenous dna of Bacillus marine atrophaeus

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Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0030] The Bacillus atrophaeus transformed into the plasmid pRP1028 in the present invention has been preserved in the China Center for Type Culture Collection (CCTCC). The address of the preservation unit is: Wuhan University, Wuhan, China, and the postal code is: 430072. The preservation date is: On May 26, 2016, the deposit number is: CCTCC M 2016269 Classification designation: Bacillus atrophaeus C89 / pRP1028Bacillus atrophaeusC89 / pRP1028.

[0031] The source of Escherichia coli in the present ...

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Abstract

The invention provides a method for transforming exogenous DNA into Marine Bacillus atrophaeus. The method comprises: respectively culturing a donor strain, an assistant strain and an acceptor strain, respectively detecting the OD600 of the donor strain culture liquid, the assistant strain culture liquid and the acceptor strain culture liquid; equally and uniformly mixing the cultured donor strain, the cultured assistant strain and the cultured acceptor strain, carrying out three-parent strain hybridization, screening the strain achieving the successful hybridization, and recording as the three-parent strain hybridization strain; screening the Bacillus atrophaeus C89 / pRP1028 having the plasmid strain; extracting the plasmid pRP1028; and transforming the plasmid pRP1028 into Escherichia coli, extracting the plasmid of the Escherichia coli, and carrying out sequencing inspection. According to the present invention, the method provides the reference significance for the transformation of the exogenous gene in the strain, wherein the strain requires the special culture and the exogenous gene is difficultly transformed into the strain.

Description

technical field [0001] The invention relates to a method for transferring exogenous DNA of bacillus marine atrophaeus, belonging to the technical field of microorganisms. Background technique [0002] Bacillus atrophaeus belongs to the genus Bacillus and forms soluble black colonies on medium containing carbohydrates. The research on the application technology of Bacillus atrophaeus is extensive and in-depth, and a large number of biologically active substances are isolated and purified from Bacillus atrophaeus, among which, the compounds neobacillamide A and bacillamide C are isolated from Bacillus atrophaeus C89 in this laboratory (Yu LL, Li ZY , Peng CS, et al. Neobacillamide A, a novel thiazole-containing alkaloid from the marinebacterium Bacillus vallismortis C89, associated with South China Sea sponge Dysidea avara. Helv Chim Acta. 2009, 92:607-612.). Bacillus atrophaeus can be used as a vaccine carrier and vaccine adjuvant, simulate biological weapons, monitor biolog...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/75C12R1/07
CPCC12N15/75
Inventor 张风丽王惟佳金结平李志勇
Owner SHANGHAI JIAOTONG UNIV
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