Chip device adopting PDMS as substrate material for subtype swine flu detection

Abstract

The invention relates to a chip device adopting PDMS as a substrate material for subtype swine flue detection, and belongs to the field of analytical tests. Polydimethylsiloxane (PDMS) which is low in price and extremely easy to process is used for making the substrate of a microfluidic chip for subtype swine flue diagnosis, and some problems actually exist; the surface of PDMS is strongly lyophobic, the effect of specific surface modification or surface decoration has difficulty in being lasting, and the invention aims at solving the series of related problems. The chip device adopting PDMS as the substrate material for subtype swine flue detection is characterized in that a substrate adopts PDMS with an original surface, a hinge type fixture additionally provided with a miniature ultrasonic transducer is manually fastened and positioned on a nearest location of a sample flow terminal of the microfluidic chip, the interfacial tension is reduced through ultrasonic waves, and meanwhile by means of a high absorption capacity of PDMS for the ultrasonic waves, the ultrasonic wave intensity is decreased progressively and rapidly within a short distance; an interfacial tension difference is formed at the two ends of the chip, and therefore a sample flow is promoted to flow along an original hydrophobic capillary channel in the terminal direction.

Description

technical field [0001] The invention relates to a subtype swine influenza detection chip device using PDMS as a substrate material. The device is a special device for diagnosing subtype swine influenza antigens based on antigen / antibody specific reaction, and belongs to the field of analysis and testing. Background technique [0002] For the technical background of multi-channel microfluidic subtype swine influenza diagnosis, please refer to CN 201110311127.1 and other invention patent applications. [0003] As far as the overall overview of microfluidic technology itself is concerned, you can refer to the monograph "Illustrated Microfluidic Chip Laboratory" published by the famous microfluidic expert Mr. Lin Bingcheng not long ago, which has been published by Science Press. The past, present, and future prospects of microfluidic technology, etc., have detailed and long-form discussions that go deep into specific details. [0004] So, let's talk about the key issues of this...

AI Technical Summary

Problems solved by technology

[0007] But it's not that simple

[0008] First, this polydimethylsiloxane material, the material referred to by the acronym PDMS, is itself a strongly hydrophobic material. Microchannels are built on this material. If the microchannels are not targeted The modification operation of the surface of the channel, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the micro channel in the structure occupies most of the inner surface of the liquid flow channel, then the PDMS micro channel The strong hydrophobic characteristic of the inner surface of the channel is the decisive factor, which will make it very difficult for the polar liquid flow similar to the aqueous solution to pass through, and its flow resistance is so large that even ordinary micropumps are difficult to push. Of course, If the cover sheet also chooses to use the PDMS material, then the problem is basically the same, with little difference; therefore, in the prior art, it is necessary to modify and modify the inner surface of the microchannel on the PDMS material; then , is this modification operation for the inner surface of the PDMS microchannel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the microchannel modified by the surface modification unable to maintain for a long enough time, and the microgroove after surface modification The maintenance time of the inner surface state of the channel is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the inner surface of the PDMS microchannel after surface modification or surface modification is formed after modification The surface state of the surface does not last long, but quickly tends to or changes back to the surface state before the surface modification, and returns to the original strongly hydrophobic surface state in a relatively short period of time. Then, just imagine, Can such microfluidic chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

This problem has also existed for many years, and so far, it has not been properly solved

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