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Method for studying reducing action of selenium-highly-resistant bacteria to selenite

A technology of selenite and sodium selenite, which is applied in the directions of microorganism-based methods, bacteria, biochemical equipment and methods, etc., to achieve the effects of simple experimental method, low material cost, and relatively high material cost.

Inactive Publication Date: 2016-11-23
ANHUI NORMAL UNIV
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  • Abstract
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Problems solved by technology

However, there are few reports about the toxicity or impact of selenium compounds on Lysinibacillus (Lysinibacillus)

Method used

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  • Method for studying reducing action of selenium-highly-resistant bacteria to selenite
  • Method for studying reducing action of selenium-highly-resistant bacteria to selenite
  • Method for studying reducing action of selenium-highly-resistant bacteria to selenite

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Embodiment Construction

[0037] In the following, the present invention will be further described in detail by describing the embodiments with reference to the accompanying drawings, so as to help those skilled in the art have a more complete, accurate and in-depth understanding of the inventive concepts and technical solutions of the present invention.

[0038] A research method for the reduction of sodium selenite by super-selenium-resistant bacteria, the method comprises the following steps,

[0039] a.Activation

[0040] Inoculate the pure culture of selenium-resistant bacteria obtained by screening into an Erlenmeyer flask with LB liquid medium, then place the inoculated Erlenmeyer flask in a shaking incubator for cultivation, the amount of the medium is 25 mL, and the shaking incubator The temperature of the incubator is 35°C, and the rotation speed of the shaking incubator is 180r min -1 , the shaking culture time is 12h;

[0041] Wherein the screening method of the pure culture of selenium-r...

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Abstract

The invention discloses a method for studying reducing action of selenium-highly-resistant bacteria to selenite. The method includes following steps: a, activating; b, performing gradient stress culture; c, counting viable bacteria; d, testing Se4+. The selenium-highly-resistant bacteria which exist in selenium-rich environment are adopted, have the characteristic of reducing sodium selenite to nanoscale red elemental selenium and have quite high development and utilization value. The method has the advantages of low material cost, simple experiment method and no environment pollution, and generated nanoscale red elemental selenium can replenish selenium of organisms.

Description

technical field [0001] The invention belongs to the technical field of selenium-tolerant microorganisms, and in particular relates to a research method for reducing sodium selenite by ultra-selenium-tolerant bacteria. Background technique [0002] Selenium is an essential trace element for many organisms. In organisms, selenium is synthesized into selenoproteins. Some selenoproteins have anti-oxidation functions, and at the same time, can prevent and treat some cancers. When the content of selenium is low, it is beneficial to organisms, and when the content is high, it is harmful to humans and animals. Among the essential trace elements for the human body, selenium has the narrowest range from deficiency to toxicity. [0003] Selenium, which is bioavailable in soil, mainly exists in the form of selenate and selenite. Agricultural drainage and semiconductor industrial wastewater in some selenium-rich areas are water-soluble selenium (selenate / selenite) in water. However, ino...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/12C12Q1/04C12N1/20C12R1/07
CPCC12Q1/12C12N1/20C12Q1/04
Inventor 杨如意李静邵宗圆苏楠楠
Owner ANHUI NORMAL UNIV
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