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Respiratory syncytial virus IgA antibody detection test strip and detection method thereof

A syncytial virus and antibody detection technology, applied in the field of medical testing, can solve problems such as nausea or vomiting, unable to collect cells, difficult diagnosis methods, etc., and achieve the effects of improving the promotion rate, simple operation, and avoiding discomfort.

Inactive Publication Date: 2016-11-16
GUANGZHOU RHFAY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology requires professional technicians and specialized fluorescent PCR amplification instruments, and it is difficult for primary medical departments to carry out this diagnostic method
Fluorescent PCR technology needs to collect cells from the patient's throat, which is more likely to cause discomfort in the patient and cause retching or vomiting
Respiratory syncytial virus is a lower respiratory tract infection, so throat swabs sometimes fail to collect virus-infected cells, resulting in missed detection

Method used

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  • Respiratory syncytial virus IgA antibody detection test strip and detection method thereof
  • Respiratory syncytial virus IgA antibody detection test strip and detection method thereof
  • Respiratory syncytial virus IgA antibody detection test strip and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] [Example 1] Preparation of Respiratory Syncytial Virus IgA Antibody Diagnostic Reagent Strips

[0026] 1. Source of raw materials: Goat anti-human IgA antibody was purchased from Sigma Company, and human IgA antibody was purchased from Guangzhou Nuosi Biotechnology Co., Ltd. The sequence of the respiratory syncytial virus fusion protein antigen polypeptide fragment is Ser Val Ile Thr Ser LeuGly Ala Ile Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn ArgGly Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val (SeqID NO.1), a total of 43 amino acids, was entrusted to Shanghai Jier Biochemical Company to be responsible for the synthesis, with a purity greater than 98%, and was stored in freeze-dried form.

[0027] 2. Preparation of goat anti-human IgA antibody colloidal gold labeling pad

[0028] In the pH 6.8-7.0 solution, the goat anti-human IgA antibody is labeled with colloidal gold particles to form a goat anti-human IgA antibody colloidal ...

Embodiment 2

[0033] [Example 2] Preparation of sterile absorbent cotton

[0034] Purchase medical absorbent cotton balls with a diameter of 2cm, and bind them with cotton threads with a length of 8-10cm. Place the treated cotton balls in a pressure cooker under the pressure condition of 121° C. for 30 minutes, place them in an oven for drying at a temperature of 100° C. for 4 hours, and vacuum-seal the dried cotton balls in an aluminum foil bag.

Embodiment 3

[0035] [Example 3] Saliva sample collection

[0036] In the present invention, the aseptic absorbent cotton is placed under the tongue of the child, the cotton thread is exposed outside the mouth, and the mouth is held for 3-5 minutes. as attached figure 2 , put the cotton ball 22 into the syringe 21, then install the syringe push rod and push it gently to obtain a saliva sample 23, which is stored in a 2ml small tube.

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Abstract

A respiratory syncytial virus IgA antibody detection test strip includes a sample pad, a labeling pad, a nitrocellulose membrane, water absorbent paper and a backing plate and is characterized in that the cellulose membrane comprises a detection line sprayed with a respiratory syncytial virus fusion protein antigen polypeptide fragment and a control line of a human IgA antibody. A saliva specimen is collected by aseptic degreasing cotton, then the saliva specimen is extruded by an injector, the IgA antibody in the saliva is detected by the respiratory syncytial virus IgA antibody rapid chromatography test strip, and the purpose of rapid diagnosis of patient infected respiratory syncytial virus is realized. The method is simple to operate, has rapid results, and is suitable for rapid diagnosis of respiratory syncytial virus infection in medical institutions at all levels. The collected specimen has no invasion, discomfort brought to patients by traditional blood sampling and nasopharyngeal swab collection is avoided, the work efficiency of health care workers is improved, and the promotion rate of respiratory syncytial virus diagnosis in clinic is improved.

Description

technical field [0001] The invention belongs to the field of medical examination, and specifically discloses a test strip for detecting IgA antibody of respiratory syncytial virus and a detection method thereof. Background technique [0002] Respiratory syncytial virus (RSV, referred to as syncytial virus, also belongs to Paramyxoviridae) is the most common pathogen causing viral pneumonia in children, which can cause interstitial pneumonia and bronchiolitis. Respiratory syncytial virus can be divided into two subtypes according to molecular biology, type A and type B. Respiratory syncytial virus is transmitted by air droplets and close contact, and the incubation period is 3 to 7 days. Infants and young children have severe symptoms, such as high fever, rhinitis, pharyngitis and laryngitis, and later manifest as bronchiolitis and pneumonia. A small number of sick children may be complicated by otitis media, pleurisy and myocarditis. The disease is more common in infants ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/558
CPCG01N33/6854G01N33/558G01N33/56983G01N2469/20
Inventor 李有生刘轶锴
Owner GUANGZHOU RHFAY BIOTECH CO LTD
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