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Occult blood detecting kit and application thereof

A technology of detection kits and detection reagents, which can be used in biological testing, material inspection products, and analysis by chemical reaction of materials, etc., can solve problems such as large gaps, low cancer survival rate, and low early diagnosis rate of early cancer, etc. To achieve the effect of simple operation, no need for special conditions and equipment, and low price

Inactive Publication Date: 2016-11-16
北京珍草中医药技术开发中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, compared with developed countries, the gap in hardware facilities between my country and developed countries is very small, but the gap in software such as prevention concept, operation technology, standardized diagnosis and treatment, and sanitary conditions is much larger, which also leads to the low rate of first diagnosis of early cancer in my country , the main reason for the low cancer survival rate

Method used

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  • Occult blood detecting kit and application thereof
  • Occult blood detecting kit and application thereof
  • Occult blood detecting kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The occult blood detection kit of the present invention includes a detection reagent, a color developing agent, occult blood beads and a cotton swab, and may also include a sputum box.

[0056] in,

[0057] (1) The detection reagent consists of the following components:

[0058] Ethanol (CH 3 CH 2 OH) 29%≤ethanol mass percentage concentration≤35%

[0059] Glacial ethanol (CH 3 COOH) 30%≤ice ethanol mass percentage concentration≤36%

[0060] Purified water 31≤purified water mass percentage concentration≤37%

[0061] Hydrogen peroxide 1% ≤ hydrogen peroxide mass percentage concentration ≤ 5%.

[0062] The preparation method of detection reagent is:

[0063] Mix ethanol, ice ethanol, purified water and hydrogen peroxide in the prescribed amount at room temperature, and stir evenly.

[0064] For example, the detection reagent can be composed of the following components in mass percentage:

[0065] Ethanol 29%, ice ethanol 36%, purified water 33%, hydrogen peroxide ...

Embodiment 2

[0080] The detection method of using the test kit of the present invention is specifically as follows:

[0081] 1. When testing, see Table 2 for the composition of the kit.

[0082] Table 2

[0083]

[0084] Among them, the "seven items" in the table include: nasal secretion, sputum, breast secretion, gastric juice, urine, vaginal and anal secretion; the "five items" include: nasal secretion, sputum, gastric juice, urine and Anal discharge.

[0085] 2. Sample collection method:

[0086] 1. Nose hair inspection: Before washing your face in the morning, take a cotton swab and put it on the nose hair area (about 1 cm deep), gently rub it for 2-3 circles, then move to the other nostril, and use the same method to gently rub it for 2-3 circles after taking out;

[0087] 2. Breast fluid inspection: For women who have fluid overflow during non-lactation period, take a cotton swab to absorb the overflow (first squeeze the breast with your hands, and then absorb the overflow); ...

Embodiment 3

[0115] Apply the kit of the present invention and the reference reagent (production batch number of "anti-cancer occult blood detection kit" of Beijing Capital Medical University Clinical Medical Science and Technology Center: 20090907) to carry out comparative experimental research, and use statistical methods to detect the consistency of the two .

[0116] Detect each 217 parts of gastric juice, anal secretion, sputum, nasal cavity, urine sample with the test kit of the present invention and reference reagent jointly, each 219 parts of nipple secretion, vaginal secretion sample, reference reagent and reagent of the present invention Box detection of various specimens showed a significant correlation.

[0117] Conclusion: There is no significant difference between the two reagents in detecting stomach, nasal cavity, sputum, anus, urine, nipple fluid and vaginal occult blood.

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Abstract

The invention provides an occult blood detecting kit which comprises a detection reagent and a color developing agent. The detection reagent is prepared from, by mass, 29-35% of ethyl alcohol, 30-36% of absolute alcohol, 31-37% of purified water and 1-5% of hydrogen peroxide. A preparation method of the detection reagent includes the steps of mixing ethyl alcohol, absolute alcohol, purified water and hydrogen peroxide at a normal temperature according to the formula amounts, and evenly stirring the materials to obtain the detection reagent. The color developing agent is guaiac resin. The invention further provides application of the occult blood detecting kit. By means of the kit, people can find the existence signals of tumors with a more-convenient method, higher sensitivity and lower price the first time when early tumors appear. That is, people can find the signals of the early tumors at least 3-10 years earlier.

Description

technical field [0001] The invention belongs to the technical field of preventive medicine, and in particular relates to an occult blood detection kit and application thereof. Background technique [0002] Today, when infectious diseases are fully controlled, the focus of global health work has shifted to the control of chronic non-communicable diseases, among which cancer prevention has been listed as the top priority of health strategies. [0003] For many years, human beings have been exploring the technology to completely conquer cancer. In recent years, gene therapy and targeted therapy have been applied in clinic successively, which have significantly improved the quality of life of cancer patients, but the overall cure rate is still limited, and cancer has not been completely conquered, but only some progress has been made in treatment technology. [0004] The World Health Organization once made such a conclusion: Today, before finding the technology and means to com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/52
CPCG01N21/78G01N33/52
Inventor 吴金秀
Owner 北京珍草中医药技术开发中心
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