Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of liquid-based cell

A liquid-based cell, cell smear technology, applied in the field of cell detection, can solve problems such as cell loss

Active Publication Date: 2016-11-16
HANGZHOU HEALTHSKY BIOTECH CO LTD
View PDF10 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a liquid-based cell sheet production method, which solves the problem of cell loss in the separation process and has the effect of reducing cell loss

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of liquid-based cell
  • Preparation method of liquid-based cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: cleaning solution, it is prepared by the following method:

[0035] Take 3g NaCl, 5g glycerin, 3g absolute ethanol and 89g double distilled water, mix them, stir evenly, filter, and take the filtrate. Under normal temperature and pressure, the density of the cleaning solution is 1.1~1.2g / cm 3 .

Embodiment 2

[0036] Embodiment 2: cleaning solution, the difference from embodiment 1 is that its formula is: 4g NaCl, 8g glycerol, 4g absolute ethanol and 84g double distilled water.

Embodiment 3

[0037] Embodiment 3: cleaning solution, the difference from embodiment 1 is that its formula is: 5g NaCl, 10g glycerol, 5g absolute ethanol and 80g double distilled water.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
densityaaaaaaaaaa
densityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of a liquid-based cell. The preparation method comprises the following steps: step 1, taking a cell smearing device, wherein the cell smearing device comprises a tray, a preparation warehouse fixed on the tray, a glass slide positioned on the lower end surface of the preparation warehouse and used for plugging the lower end of the preparation warehouse, a filter membrane detachably arranged in the preparation warehouse and arranged in the circumferential direction of the preparation warehouse, a filter chamber formed in the preparation warehouse and positioned above the filter membrane and a cleaning chamber formed in the preparation warehouse and positioned under the filter membrane; adding cleaning liquid into the preparation warehouse and enabling all the cleaning liquid to enter the cleaning chamber and the liquid level of the cleaning liquid to be flush with the filer membrane; step 2, taking and adding a mixture of a sample and a preservation solution into the filter chamber, enabling the mixture to pass through the filter membrane to enter the cleaning chamber and enabling a cell to settle to the bottom of the cleaning chamber; step 3, removing the filter membrane as well as impurities on the filter membrane and enabling natural settlement to remove liquid supernatant; adding cleaning liquid, centrifuging and abandoning liquid supernatant; step 4, extracting the glass slide, fixing, dying and sealing. The preparation method has the effect of reducing the cell loss.

Description

technical field [0001] The invention relates to the field of cell detection, in particular to a liquid-based cell sheet preparation method. Background technique [0002] Liquid-based cytology examination is to use liquid-based thin-layer cytology detection system to detect cervical cells and carry out cytological classification diagnosis. Compared with the traditional cervical smear and Pap smear, liquid-based cytology has significantly improved the satisfaction of samples and the detection rate of abnormal cervical cells. Liquid-based cytology is currently a relatively advanced cervical cancer in the world. Cytology techniques. [0003] The Chinese patent with application publication number CN102680290A and application publication date of September 19, 2012 discloses a method for automatically producing liquid-based cell slides on a production device. The method includes: a. separating cells , use a cell breaker to repeatedly extract and discharge the cell fluid, and brea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
Inventor 毛立新陈志俊王云皓项伟平
Owner HANGZHOU HEALTHSKY BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com