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MiRNA molecular marker hsa-miR-149-3p for diagnosing type 2 diabetes mellitus and application thereof

A technology of molecular markers and type 2 diabetes, applied in the field of biomedicine, can solve the problems of invasiveness and limitations of research methods, and achieve the effect of less invasiveness, obvious clinical application value, and high sensitivity

Active Publication Date: 2016-11-16
HENAN UNIVERSITY
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Problems solved by technology

At present, a large number of studies have shown that miRNA is involved in the pathogenesis of diabetes from various stages such as islet development, islet cell apoptosis, insulin production, secretion, and insulin action, but the research methods are mostly limited to animal models or diseased tissues, and need to be passed. traumatic way to get

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  • MiRNA molecular marker hsa-miR-149-3p for diagnosing type 2 diabetes mellitus and application thereof
  • MiRNA molecular marker hsa-miR-149-3p for diagnosing type 2 diabetes mellitus and application thereof
  • MiRNA molecular marker hsa-miR-149-3p for diagnosing type 2 diabetes mellitus and application thereof

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Embodiment Construction

[0021] The present invention is further described by the following examples, including materials used and specific sources. But it should be understood that these are just examples, not limitations of the present invention. Materials similar or identical to the types and models of the following reagents and instruments, or properties or functions can be used in the practice of the present invention. Unless otherwise indicated, the reagents used in the present invention may be any suitable commercially available reagents.

[0022] The methods in the following examples are common methods unless otherwise specified.

[0023] Main material:

[0024]

[0025] 1. Collection of clinical samples

[0026] The inventor has collected a large number of peripheral blood samples from patients with type 2 diabetes and people with normal physical examinations from the Huaihe Hospital Affiliated to Henan University since 2015. The entire collection and follow-up experiment process confo...

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Abstract

The invention belongs to the field of biomedicine and relates to a miRNA molecular marker for diagnosing type 2 diabetes mellitus and application thereof. The steps are as follows: extraction of total RNA in blood and preparation of cDNA; fluorescent quantitative PCR detection of hsa-miR-149-3p level; evaluation of the miRNA molecular marker. According to the invention, large sample tests indicate that novel miRNA marker hsa-miR-149-3p in peripheral blood is significantly increased in the type 2 diabetes mellitus, and has a molecular level positively correlated with the blood glucose level of a subject. A detection method is simple and easy to operate, has little trauma to the subject, is high in sensitivity and specificity, and can be used for the diagnosis of type 2 diabetes mellitus at early stage and different stages. On this basis, the invention develops a primer, a kit and the detection method for detecting hsa-miR-149-3p in the blood, and has obvious clinical application value.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a miRNA molecular marker for diagnosing type 2 diabetes and its application. Background technique [0002] Diabetes mellitus is a chronic metabolic disease characterized by hyperglycemia. The complications caused by persistent hyperglycemia will seriously damage the life and health of patients. According to the latest data from the International Diabetes Federation, in 2015, the number of adults with diabetes in the world was nearly 400 million, and the prevalence in my country accounted for about 1 / 4, which brought an increasingly heavy burden to society and families. Diabetes is mainly divided into type 1 and type 2 diabetes according to the pathogenesis. Type 2 diabetes, also known as adult-onset diabetes, accounts for about 94% of diabetes. Type 2 diabetes cannot be completely cured, and there are no obvious clinical symptoms in the early stage. Patients are often diagnosed only aft...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/178C12Q2600/158C12N15/113C12N2310/141A01K2227/105A01K2207/25A01K2267/0362A61K31/7088A61K48/00
Inventor 陈卫东聂小博王艳东周云吕心瑞
Owner HENAN UNIVERSITY
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