Method for lowering leaching toxicity of waste incineration fly ash through bacillus firmus
A technology for waste incineration fly ash and Bacillus firmus, applied in chemical instruments and methods, solid waste removal, transportation and packaging, etc., to achieve the effects of reducing leaching toxicity, good heavy metal stabilization effect, and good environmental adaptability
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Embodiment 1
[0027] Step 1) Purchasing Bacillus firmus strains at China General Microorganism Culture Collection Center (CGMCC);
[0028] Step 2) prepare culture medium
[0029] The composition of the culture medium is shown in Table 1. It was sterilized at a high temperature of 121° C. and a high pressure of 0.15 MPa for 20 minutes, and then allowed to stand at room temperature.
[0030] Table 1 Medium composition
[0031] composition
Mass / g
effect
Peptone
5.0
meat extract
3.0
Nutrients
20.0
Inorganic salt
1000.0
Provide moisture, growth environment
[0032] Step 3) bacterial culture and bacterial concentration measurement
[0033] Inoculate the bacterial species in step 1) into a Erlenmeyer flask containing medium at a 1% (v / v) inoculum amount on an ultra-clean bench, and vibrate and culture at 30°C and 220r / min on a shaker for 46h.
[0034] Bacteria concentratio...
Embodiment 2
[0041] Step 1) Purchasing Bacillus firmus strains at China General Microorganism Culture Collection Center (CGMCC);
[0042] Step 2) prepare culture medium
[0043] The composition of the medium is shown in Table 2. It was sterilized at 121° C. under high pressure of 0.15 MPa for 15 minutes, and allowed to stand at room temperature.
[0044] Table 2 Medium composition
[0045] composition
Mass / g
effect
Peptone
3.0
meat extract
1.0
Nutrients
15.0
Inorganic salt
600.0
Provide moisture, growth environment
[0046] Step 3) bacterial culture and bacterial concentration measurement
[0047] On the ultra-clean bench, inoculate the bacterial species in step 1) into the Erlenmeyer flask containing the culture medium according to the inoculum amount of 1% (v / v), and shake and culture at 20° C. and 240 r / min on a shaker for 24 hours.
[0048] Bacteria concentrati...
Embodiment 3
[0054] Step 1) Purchase a sample of Bacillus firmus at the China General Microorganism Culture Collection Center (CGMCC);
[0055] Step 2) prepare culture medium
[0056] The composition of the culture medium is shown in Table 3. It was sterilized at a high temperature of 121° C. and a high pressure of 0.15 MPa for 20 minutes, and then allowed to stand at room temperature.
[0057] Table 3 Medium composition
[0058] composition
Mass / g
effect
Peptone
7.0
meat extract
5.0
Nutrients
Sodium chloride
30.0
Inorganic salt
1400.0
Provide moisture, growth environment
[0059] Step 3) bacterial culture and bacterial concentration measurement
[0060] Inoculate the bacterial species in step 1) into a Erlenmeyer flask containing a culture medium at a 1% (v / v) inoculum amount on a clean bench, and vibrate and culture at 35° C. and 180 r / min on a shaker for 48 hours.
[0061] Bact...
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