Method for preparing oyster oligopeptide by utilizing fresh oyster meat

Abstract

The invention discloses a method for preparing oyster oligopeptide by utilizing fresh oyster meat. The method for preparing the oyster oligopeptide by utilizing the fresh oyster meat specially comprises the following steps: taking fresh oyster meat, and carrying out crushing and pulping so as to obtain an oyster pulp; adding water into the oyster pulp, and then, heating the mixture to 40-55 DEG C and adjusting the pH value to 2.0-5.0; adding compound proteases so as to carry out enzymatic hydrolysis, and then, raising the temperature to 80-95 DEG C so as to deactivate the enzymes; carrying out centrifugal separation, and collecting the liquid phase; separating the liquid phase by adopting nano-filtration membrane separation, and collecting the permeate liquid; performing electroosmosis on the permeate liquid so as to carry out desalination; adding beta-cyclodextrin and activated carbon into the desalinated liquid so as to carry out refining, carrying out filtrating by using a plate-and-frame filter so as to decarbonize, and collecting the liquid phase, so that a refined liquid is prepared; concentrating the refined liquid by using a membrane concentration device, and then, carrying out spray-drying, so that the oyster oligopeptide powder is prepared. The method for preparing the oyster oligopeptide by utilizing the fresh oyster meat disclosed by the invention increases added values of the oysters. The prepared products are high in oyster oligopeptide contents, and have molecular weights of 1000 Da or lower; thus, the oyster oligopeptide can be completely absorbed by the human body. The method for preparing the oyster oligopeptide by utilizing the fresh oyster meat has extremely important realistic meanings in promoting the rapid developments of the marine industry of China.

Description

technical field [0001] The invention relates to the field of oyster oligopeptide preparation. More specifically, it relates to a method for preparing oyster oligopeptides by enzymatically hydrolyzing oyster meat with fresh oyster meat. Background technique [0002] Oyster is the largest cultured shellfish in the world, and it is also one of the four famous economical shellfish cultured in my country. Oyster software is rich in protein, glycogen, taurine, amino acids and other nutrients, and has great edible and medicinal value. Peptides are intermediate products of protein hydrolysis, generally, those consisting of 2 to 10 amino acids are called oligopeptides, and those consisting of 11 to 50 amino acids are called polypeptides. In terms of material composition, peptides have homogeneity with proteins, but have unique physiological functions different from amino acids and proteins, especially oligopeptides. Scientific research shows that the main form of protein absorbed ...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, oysters are used as raw materials to obtain oyster peptides through enzymatic hydrolysis. The obtained peptides have a large molecular weight, are not easily absorbed by the human body, and have a bitter and fishy smell, and the taste is extremely poor