Tissue culture and rapid propagation method for spathiphyllum

Abstract

The present invention belongs to the field of plant tissue culture, and specifically relates to a tissue culture and rapid propagation method of white crane lily. Sterilized with 0.1% mercuric chloride, ②cut the stems into 1-2mm thick slices, and put them together with the terminal buds in the induction and differentiation medium supplemented with 6‑BA and VC for culture, ③callus or adventitious buds were inoculated Add 6‑BA and IAA to the medium, wherein the adventitious bud mass used to induce rooting seedlings is directly inserted into the medium added 6‑BA, IAA and TIBA, ④Single buds with plant height ≥30mm or Three clumps with a main bud plant height ≥ 25mm were inserted into the rooting medium supplemented with activated carbon. The invention provides a method for tissue culture and rapid propagation of white crane lily, which can obtain a large amount of asexually propagated white crane lily tissue culture seedlings in a short time.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and relates to a method for tissue culture and rapid propagation of white crane lily. Background technique [0002] White crane lily is a perennial evergreen herbaceous plant of the family Araceae. The emerald-green leaves and pure white spathe are very fresh and elegant. It is one of the most popular ornamental flowers in the world today and has high ornamental value and economic value. [0003] At present, the methods of propagation of Craneus chinensis mainly include division, sowing and tissue culture. [0004] Rite propagation is generally carried out in May to June. The vigorously growing plants can be divided once every two years. The reproduction coefficient is extremely low and it is difficult to expand on a large scale. [0005] Sowing and breeding, white crane taro is difficult to bear fruit in the natural state, and it needs to be artificially pollinated to get seeds. After the s...

AI Technical Summary

Problems solved by technology

The rapid propagation model using leaves, petioles, filament somatic cells, and inflorescence somatic cells as explants is still in the laboratory stage, and the induction effect on leaves and petioles is not ideal. The callus takes a long time and the degree of callus Low, not suitable for large-scale production

The technique of inducing filament somatic cells and inflorescence somatic cells is difficult and requires micromanipulation. Once the micromanipulation is inadvertent, it is very easy to induce plants of different genotypes, and it is not suitable for large-scale production.