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Sensor based on singlet oxygen channel light-emitting quantum dots

A technology of emitting quantum and singlet oxygen, which is applied in the field of immunodetection, can solve the problems such as the large limitation of FRET transmission distance, which cannot exceed 10nm, and the requirement of near-infrared sensitive detectors still exist.

Inactive Publication Date: 2016-11-09
SOUTHERN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, the FRET transmission distance is limited, and generally cannot exceed the spatial distance of 10nm
On the other hand, due to the broad fluorescence emission spectrum of most organic dyes and obvious tailing, the existing HTRFA technology is only suitable for analysis in the long-wavelength or near-infrared band, and the requirement for expensive near-infrared sensitive detectors still exists

Method used

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Embodiment 1

[0048] The core technology of this step is to obtain symmetrical water-soluble quantum dots with high quantum yield and narrow maximum half-maximum width of the fluorescence emission peak. The concrete implementation of the present invention used is the core / shell type cadmium selenide / zinc sulfide quantum dot purchased from U.S. Invitrogen Company, and its solvent is decane, and its spectral Stock shift length is from 592nm to the wider range between short wavelength Therefore, the quantum dot can be excited with a wavelength shorter than 592nm; the fluorescence emission peak is 605±0.5nm, the maximum half-peak width is 27±1.5nm, and the quantum yield is 76%; Oxygen-receiving "antenna" thiazine compound has a matching fluorescence emission spectrum, overcomes the shortcomings of organic dyes in all aspects, and is suitable for replacement to reduce measurement errors, improve fluorescence resonance energy transfer (FRET) efficiency, and improve analytical sensitivity.

[0049...

Embodiment 2

[0059] A luminescent quantum dot sensor based on a singlet oxygen channel, comprising quantum dot nano hydrosol and functionalized photosensitive microspheres; the preparation method of quantum dot nano hydrosol and functionalized photosensitive microspheres is as follows:

[0060] 1. Preparation of high-performance water-soluble quantum dots: put 50 μL of fat-soluble quantum dots in a centrifuge tube, add 200 μL of anhydrous methanol; mix well, seal the centrifuge tube, centrifuge at 3000 rpm, 4 ° C for 5 min; discard the supernatant Add 40 μL of chloroform to fully dissolve; add 21 mg of glutathione stabilizer and 10 μL of 0.1M sodium hydroxide aqueous solution, mix well, shake and incubate on a shaker, and store at room temperature for 2 hours; add 100 μL of deionized water and 1.8 mL of acetone, precipitated, centrifuged at 6000 rpm for 5 min; dissolved in 0.5 mL of deionized water to obtain water-soluble quantum dots. The emission spectrum of the prepared water-soluble qu...

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Abstract

The invention discloses a sensor based on singlet oxygen channel light-emitting quantum dots. The sensor comprises quantum dot nano hydrosol and functional photosensitive microspheres; the quantum dot nano hydrosol modifies the fat-soluble quantum dots with the emission wavelength being 520-620 nm to the water-soluble quantum dots;` the water-soluble quantum dots and a thiazine compound are coated by nano latex spheres which are modified by carboxyl, amino, hydroxyl, aldehyde group or sulfo group to prepare the quantum dot nano latex spheres; surface of the quantum dot nano latex spheres is modified with glucan gel to obtain the quantum dot nano hydrosol; the functional photosensitive microspheres are characterized in that a photosensitive material phthalocyanine compound is coated by the nano latex spheres which are modified by carboxyl, amino, hydroxyl, aldehyde group or sulfo group, and the functional photosensitive microspheres can be obtained. The sensor is a novel homogeneous fluoroimmunoassay sensor based on the singlet oxygen transmission, a one-to-one mode of a traditional energy ligand is changed, and a break through is provided to the space detection distance with 20 nm.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a singlet oxygen channel-based luminescent quantum dot sensor. Background technique [0002] Carcinoembryonic antigen (CEA) is a glycoprotein complex with a molecular weight of about 220KDa. It was first discovered in colon cancer tissue extracts by Canadian scientists Samuel O. Freedman and Phil Gold in 1965. Generally speaking, carcinoembryonic antigen is synthesized by gastrointestinal tract tissue, pancreas and liver cells during fetal period, and basically ceases to be synthesized after birth. Therefore, the content of carcinoembryonic antigen in the serum of normal healthy individuals is maintained at a very low level, about 2.5 μg / mL. It is generally believed that elevated levels of CEA in serum are more common in patients with gastric cancer, pancreatic cancer, lung cancer, breast cancer, and medullary thyroid cancer, and are also common in some non-neoplastic d...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/544G01N33/543G01N33/533G01N21/64
CPCG01N33/57473G01N21/64G01N33/533G01N33/54313G01N33/544
Inventor 刘天才陈振华
Owner SOUTHERN MEDICAL UNIVERSITY
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