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Application of GPS2 gene in preparation of drugs for prognosis, diagnosis or prevention and treatment of soft tissue tumor

A technology for soft tissue sarcoma, which is applied in the field of biomedicine, and can solve the problem of no effective means for the prognosis of the treatment effect of patients

Active Publication Date: 2016-11-09
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no effective means for the prognosis of the patient's treatment effect

Method used

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  • Application of GPS2 gene in preparation of drugs for prognosis, diagnosis or prevention and treatment of soft tissue tumor
  • Application of GPS2 gene in preparation of drugs for prognosis, diagnosis or prevention and treatment of soft tissue tumor
  • Application of GPS2 gene in preparation of drugs for prognosis, diagnosis or prevention and treatment of soft tissue tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] Example 1: Study on the expression level of GPS2 in liposarcoma and normal tissue

[0121]The present inventors isolated RNA from tumor tissues of 14 cases of liposarcoma patients after surgery (postoperatively diagnosed as liposarcoma by the Pathology Department of 301 Hospital, and clarified the differentiation type) and the same patient's own normal subcutaneous fat tissue. The expression of GPS2 mRNA was detected by real-time quantitative PCR, and the results proved that the expression of GPS2 gene in tumor tissue was significantly lower than that in control normal tissue (P<0.05). The present inventor also analyzed the low expression of GPS2 protein in liposarcoma tissue by immunohistochemical research method.

[0122] The specific operation is as follows:

[0123] 1. Experimental materials, reagents and experimental objects

[0124] 1.1qRT-PCR

[0125] Experimental materials and reagents:

[0126] 1) Mortar, RNase free EP tube, Trizol, ethanol, DEPC water, c...

Embodiment 2

[0208] Example 2: Research on reducing the level of GPS2 gene to promote the proliferation of liposarcoma cells

[0209] 1. Experimental materials, reagents and experimental objects

[0210] 1.1 Western Blotting detection

[0211] Experimental materials: 30% acrylamide, nitrocellulose membrane, transfer solution, TBST, 10% skimmed milk powder, primary and secondary antibodies (diluted in TBST), enhanced luminescence detection kit (ECL, Amersham Pharmacia), Tanon 5200 imaging system (Tanon Co., Ltd., Shanghai, China).

[0212] 1.2 DYE670 dyeing

[0213] Experimental material: Cell Proliferation Dye 670 (eBioscience, USA), DMEM medium containing 10% FBS, DMEM medium containing 5% FBS, trypsin, FACS flow cytometer (BD, Calibur, USA).

[0214] 1.3 BrdU staining

[0215] BrdU kit (KGI Biotechnology Co., Ltd., Nanjing, China), inverted microscope imaging (Olympus, Hamburg, Germany), DMEM medium containing 10% FBS.

[0216] 1.4 The concentration of glucose in the supernatant...

Embodiment 3

[0256] Example 3: Research on Effecting the Cell Cycle and Apoptosis of Liposarcoma Cells After Lowering the GPS2 Gene

[0257] 1. Experimental materials and reagents

[0258] 1.1 Cell apoptosis experiment materials: cell apoptosis kit (Sanjian Biotechnology Co., Ltd., Tianjin, China), DMEM medium containing 10% FBS, and serum-free DMEM medium.

[0259] 1.2 Cell cycle experiment materials: cell cycle detection kit (Sanjian Biotechnology Co., Ltd., Tianjin, China), PBS, PBS containing 5% FBS, absolute ethanol, trypsin, FACS flow cytometer (BD, Calibur, United States).

[0260] 2. Experimental method

[0261] 2.1 Apoptosis experiment method:

[0262] 1) Collect SW872 cells infected with Sh-ctrl and Sh-GPS2 by trypsinization, resuspend with normal medium at 3×105 cells / well, and spread them into 6-well plates;

[0263] 2) After the cells adhered to the wall, they were replaced with serum-free DMEM medium and cultured for 24h and 48h;

[0264] 3) Wash the cells twice with t...

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Abstract

The invention belongs to the field of biological medicines and relates to an application of a GPS2 gene in preparation of drugs for prognosis, diagnosis or prevention and treatment of soft tissue tumor. The GPS2 gene is in low expression in soft-tissue sarcoma such as liposarcoma, and proliferation of the liposarcoma can be promoted by inhibiting the expression of the GPS2 gene. The expression of the GPS2 gene has obvious relevance with prognosis of the liposarcoma, which shows that the GPS2 gene can be used for clinical pathological diagnosis of patients with liposarcoma. The GPS2 gene can be used as a tumor-suppressing gene in the liposarcoma, and can be used as a possible prognostic marker and a therapeutic molecule of the liposarcoma.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the use of GPS2 for preparing drugs for the prognosis, diagnosis or prevention of soft tissue tumors. Background technique [0002] Liposarcoma (LPS) is the most common type of soft tissue sarcomas, accounting for approximately 20% of sarcomas in adults. However, the molecular mechanisms underlying the pathogenesis of this malignancy have not been fully elucidated. Over the past few decades, new approaches, including gene expression profiling, DNA amplification profiling, whole genome sequencing, miRNA profiling, and RNA sequencing, have successfully expanded the understanding of liposarcoma pathogenesis. Amplification of chromosome 12 q14-15 is the most studied molecular alteration in liposarcoma, which can lead to amplification of MDM2 (at 12q15) and CDK4 genes in nearly 90% of well-differentiated / undifferentiated liposarcoma cases. This expansion has been reported to cause a decrease...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K38/16C12Q1/68G01N33/68G01N33/574A61P35/00
CPCA61K38/16A61K48/0058C12Q1/6886C12Q2600/118C12Q2600/156C12Q2600/158G01N33/57407G01N33/68
Inventor 王立生肖凤君吴祖泽杨晓明黄晓东尹荣华王华杨月峰
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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