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Application of rat intestinal extract in preparation of bronchial smooth muscle relaxation medicines

A technique for tracheal smooth muscle and extract, applied in the field of medicine, can solve the problems of no research report on the application of rat intestinal extract, etc., and achieve the effects of simple preparation method, low cost, and relaxation of tracheal smooth muscle contraction.

Inactive Publication Date: 2016-11-09
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern studies have found that animal tissue extracts also have new effects such as treating skin burns, eliminating stretch marks, and treating vitiligo. However, there is no research report on the application of rat intestinal extracts in the preparation of tracheal smooth muscle relaxation drugs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation method of rat intestinal extract

[0024] (1) the rats are fasted, and the rats are anesthetized by intraperitoneal injection of pentobarbital sodium;

[0025] (2) Rats were killed by cervical dislocation, and the abdomen was quickly opened with sterilized scissors, and the intestinal tract 13 cm below the initial gastric pylorus was taken out along the mesenteric border, and put into high calcium solution to remove the intestinal mesentery and Peripheral adipose tissue was cut open and soaked in 5 mL of phosphate buffer solution at 4°C for 30 minutes to obtain the extract;

[0026] (3) Filter the extract with a 0.22 μm filter membrane, and adjust the filtrate to pH 7.40 with 5 mol / L sodium hydroxide solution to obtain rat intestinal extract.

[0027] The preparation of the high calcium solution: get 7.889g of sodium chloride, 0.3728g of potassium chloride, 1.982g of glucose, 2.383g of 4-hydroxyethylpiperazineethanesulfonic acid, 0.2033g of magnes...

Embodiment 2

[0028] Example 2 Preparation method of rat intestinal extract

[0029] (1) the rats are fasted, and the rats are anesthetized by intraperitoneal injection of pentobarbital sodium;

[0030] (2) Rats were killed by cervical dislocation, and the laparotomy was quickly performed with sterilized scissors. The intestinal tract was separated along the edge of the mesentery and put into a high-calcium solution to remove the intestinal mesentery and surrounding fat tissue, and then the intestinal tract was cut open. Soak in 10 mL of phosphate buffer solution at 2°C for 45 minutes to obtain the extract;

[0031] (3) Filter the extract with a 0.22 μm membrane filter, and then adjust the filtrate to pH 7.40 with 3 mol / L sodium hydroxide solution to obtain rat intestinal extract.

Embodiment 3

[0032] Example 3 Preparation method of rat intestinal extract

[0033] (1) the rats are fasted, and the rats are anesthetized by intraperitoneal injection of pentobarbital sodium;

[0034] (2) Rats were killed by cervical dislocation, and the laparotomy was quickly performed with sterilized scissors. The intestinal tract was separated along the edge of the mesentery and put into a high-calcium solution to remove the intestinal mesentery and surrounding fat tissue, and then the intestinal tract was cut open. Soak in 8 mL of phosphate buffer solution at 0°C for 60 min to obtain the extract;

[0035] (3) Filter the extract with a 0.22 μm membrane filter, and then adjust the filtrate to pH 7.40 with 3 mol / L sodium hydroxide solution to obtain rat intestinal extract.

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PUM

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Abstract

The invention relates to an application of a rat intestinal extract in preparation of bronchial smooth muscle relaxation medicines. A preparation method of the rat intestinal extract comprises the following steps of anesthetizing a rat after food forbidding, fetching out intestinal traces, soaking into 5-10mL of phosphate buffer solution for 30-60min at the temperature of 0-4 DEG C, filtering an extracting liquid by a 0.22mum filter membrane, and adjusting the filtrate by a sodium hydroxide solution to pH (potential of hydrogen) value of 7.40. The bronchial smooth muscle relaxation medicine prepared by the rat intestinal extract has the advantages that the pharmacologic action is good, the contraction of bronchial smooth muscles caused by acetyl choline can be effectively relaxed, and a novel asthma treatment medicine is provided for clinical application.

Description

technical field [0001] The invention relates to a rat intestinal extract and its application, in particular to the application of the rat intestinal extract in the preparation of tracheal smooth muscle relaxing drugs, which belongs to the technical field of medicine. Background technique [0002] Asthma is a highly sensitive disease of the airway caused by external or certain allergens present in the human body. Extensive reversible spasm and stenosis of the trachea due to oversensitivity of the trachea to irritants. The clinical manifestation is paroxysmal dyspnea with wheezing, which lasts for several minutes to several hours, and improves after treatment or spontaneously. [0003] At present, the drugs for the treatment of asthma are divided into controlling drugs and relieving drugs. Controlling drugs refer to drugs that require long-term regular daily use. These drugs mainly maintain clinical control of asthma through anti-inflammatory effects, including inhaled corti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/38A61P11/06
CPCA61K35/38
Inventor 刘庆华王倩于孟飞
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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