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Preparation method of Corbicula fluminea polypeptide chelated zinc

A technology for chelating zinc and clam, which is applied to the functions of food ingredients, food science, food ingredients, etc., can solve problems such as hindering the absorption of nutrients, and achieve good chelation effects, increased activity, and good biological activity.

Active Publication Date: 2016-11-09
JIANGSU LONGLIQI BIOSCIENCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plus, the supplement is high in zinc, which can hinder the absorption of other nutrients like calcium and iron

Method used

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  • Preparation method of Corbicula fluminea polypeptide chelated zinc
  • Preparation method of Corbicula fluminea polypeptide chelated zinc
  • Preparation method of Corbicula fluminea polypeptide chelated zinc

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Take the bagged frozen clam meat and thaw it under running water at room temperature. Wash the thawed clam meat to remove impurities such as sediment, squeeze dry and weigh. Put the river clam meat into a high-speed tissue grinder and add pure water to homogenize it, which is the river clam homogenate.

[0037] (2) Add 2.0% neutral protease according to the enzyme activity to the above clam homogenate, based on the quality of the clam homogenate, and enzymolyze it in a water bath at 56°C for 4 hours.

[0038] (3) After enzymatic hydrolysis, keep it in a constant temperature water bath at 100° C. for 20 minutes to inactivate the enzyme.

[0039] (4) Cool down after inactivating the enzyme, and perform centrifugation in a high-speed centrifuge at a speed of 4000r / min for 30 minutes to remove the precipitate, and the obtained supernatant is the clam enzymatic hydrolysis solution.

[0040] (5) Vacuum freeze-dry the above-mentioned clam enzymatic hydrolyzate for 10 h t...

Embodiment 2

[0048] (1) Take the bagged frozen clam meat and thaw it under running water at room temperature. Wash the thawed clam meat to remove impurities such as sediment, squeeze dry and weigh. Put the river clam meat into a high-speed tissue grinder and add pure water to homogenize it, which is the river clam homogenate.

[0049] (2) Add 1.0% neutral protease to the above clam homogenate based on the quality of the clam homogenate, and hydrolyze it in a water bath at 56°C for 4 hours.

[0050] (3) After enzymatic hydrolysis, keep it in a constant temperature water bath at 100° C. for 20 minutes to inactivate the enzyme.

[0051] (4) Cool down after inactivating the enzyme, and perform centrifugation in a high-speed centrifuge at a speed of 4000r / min for 30 minutes to remove the precipitate, and the obtained supernatant is the clam enzymatic hydrolysis solution.

[0052] (5) Vacuum freeze-dry the above-mentioned clam enzymatic hydrolyzate for 10 h to prepare the clam polypeptide powd...

Embodiment 3

[0060] (1) Take the bagged frozen clam meat and thaw it under running water at room temperature. Wash the thawed clam meat to remove impurities such as sediment, squeeze dry and weigh. Put the river clam meat into a high-speed tissue grinder and add pure water to homogenize it, which is the river clam homogenate.

[0061] (2) Add 1.0% neutral protease to the above clam homogenate based on the quality of the clam homogenate, and hydrolyze it in a water bath at 56°C for 4 hours.

[0062] (3) After enzymatic hydrolysis, keep it in a constant temperature water bath at 100° C. for 20 minutes to inactivate the enzyme.

[0063] (4) Cool down after inactivating the enzyme, and perform centrifugation in a high-speed centrifuge at a speed of 4000r / min for 30 minutes to remove the precipitate, and the obtained supernatant is the clam enzymatic hydrolysis solution.

[0064] (5) Vacuum freeze-dry the above-mentioned clam enzymatic hydrolyzate for 10 h to prepare the clam polypeptide powd...

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Abstract

The invention discloses a preparation method of Corbicula fluminea polypeptide chelated zinc with a high chelating rate. The preparation method comprises the steps of: 1) preparing Corbicula fluminea homogenate; 2) adding neutral protease, and performing enzymolysis in constant temperature water bath; 3) conducting enzyme deactivation after enzymolysis; 4) carrying out centrifugal treatment to remove precipitate so as to obtain supernatant, i.e. Corbicula fluminea enzymolysis liquid; 5) subjecting the Corbicula fluminea enzymolysis liquid to vacuum freeze drying so as to obtain Corbicula fluminea polypeptide powder; 6) preparing a Corbicula fluminea polypeptide solution; 7) adding zinc vitriol and the Corbicula fluminea polypeptide solution (with a pH value of 5.06-6) to perform chelation; 8) carrying out two-stage chelation reaction; 9) then conducting chelation reaction; 10) removing the supernatant and collecting the precipitate; and 11) conducting vacuum freeze drying to obtain Corbicula fluminea polypeptide zinc chelate powder. The method provided by the invention can achieve good chelating effect, has high chelating rate up to 81% or more, the oxidation resistance of Corbicula fluminea polypeptide is significantly improved after chelating with zinc, and compared with polypeptide, the chelate has better bioactivity.

Description

technical field [0001] The invention relates to a preparation method of clam polypeptide chelated zinc, which belongs to the technical field of biological products. Background technique [0002] River clam (Corbicula fluminea) is a kind of bivalve mollusk aquatic organism with strong environmental adaptability. It spreads all over the country, is widely distributed, and has abundant resources and high output. River clams usually live in brackish water and fresh water. They grow very fast and have strong reproductive ability. River clam has high nutritional value and can be eaten. It is a kind of food with both medicine and food. [0003] Clam meat has sufficient nutrients and also contains a full range of amino acids. Its fat is high in quality, rich in EPA and DHA, and also contains calcium, sodium, potassium, magnesium, iron, zinc, copper, manganese and other inorganic salts . Most of the nutrients it contains are the nutrients needed by the human body, and it can bette...

Claims

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Application Information

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IPC IPC(8): A23L33/165A23L33/18
CPCA23V2002/00A23V2200/30
Inventor 刘晶晶张颖程润青杜瑜欣张蕾韩曜平王雪锋戴阳军
Owner JIANGSU LONGLIQI BIOSCIENCE CO LTD
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