A novel circulating tumor cell detecting method based on a chiral nanometer assembly
A nano-assembly, tumor cell technology, applied in color/spectral property measurement, measurement devices, instruments, etc., can solve the problem of less research on plasma chirality
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[0045] 1) Preparation of Ag@Au core-shell nanoparticles
[0046] Silver nanoparticles with a particle size of 12±3nm were synthesized according to the previously reported method, 1 mL was centrifuged at 16200 g for 10 minutes, concentrated 10 times, and redissolved in 100 μL ultrapure water. 100 μL of the silver nanoparticle solution was added to 1 mL of a 1% polyvinylpyrrolidone solution under uniform stirring, and then 100 μL of a 100 mM ascorbic acid solution was added thereto. The pH of the solution was then adjusted to 9.5. A 0.1 mM chloroauric acid solution was added to the mixed solution at a rate of 100 μL / min using a constant flow pump. After stirring for 1 hour the final product solution was centrifuged and redissolved in 100 μL of ultrapure water.
[0047] 2) Preparation of HER2 aptamer-modified chiral Ag@Au core-shell nanoparticle assemblies
[0048] In a phosphate buffer solution containing 20 mM sodium chloride, the prepared Ag@Au NPs were modified with DNA by...
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