Method for measuring soil denitrification contribution rate of soil microbes

Abstract

The invention provides a method for measuring the soil denitrification contribution rate of soil microbes. The method comprises the following steps: picking a sample field in an ecological system, in the sample field, adopting a wild quincunx sampling method to pick 10 small units, carrying out a root breaking and litter removing treatment, entrenching around each small unit, inserting fiber glass to prevent inward growing of root, filling the soil back to the trenches; at the same time, cleaning the litters on the ground, fixing a nylon screen frame above each small unit to ensure that no root or litter exists in the small units; finally utilizing a 15 N isotope direct measuring method, which has a high sensitivity and does not need to disturb the soil, to measure the denitrification rate of in-situ processed soil in a small quadrat in the field, at the same time, measuring the denitrification rate of soil in a reference quadrat, and according to a fitting equation, calculating the soil denitrification contribution rate of soil microbes. The provided method has an important meaning for the research that whole soil microflora participates in global biochemical nitrogen circulation.

Description

technical field [0001] The invention relates to the field of biogeochemistry, in particular to a method for measuring the contribution rate of soil microorganisms to soil denitrification. Background technique [0002] Soil denitrification is an important part of the biogeochemical nitrogen cycle. Under the action of soil microorganisms, the nitrate in the soil is reduced to nitrogen oxides and nitrogen gas; the N produced in its biochemical process 2 and various nitrogen oxides such as NO, N 2 O, not only a major way of nitrogen loss, but also pollutes the atmosphere. In addition, N 2 O is a greenhouse gas, after entering the atmosphere, it will make O 3 The protective layer is destroyed and directly endangers the atmosphere and the biosphere. At the same time, N 2 O can also produce secondary chemical reactions with organic chemical components in the atmosphere to become the main source of smog, pollute the atmosphere, and bring harm to human life. According to a stud...

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Problems solved by technology

[0004] In existing techniques, only soil release of N 2 O greenhouse gas is used to study the regulation of carbon and nitrogen balance in the soil ecosystem, and the determination of the contribution rate of soil microorganisms to the soil denitrification process without the interaction of the root-microbe-litter complex system is currently not intuitively determined.

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