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Germinating method of Euonymus schensianus seeds

A seed and umbilical technology, applied in the agricultural field, can solve the problems of hard seedlings not easy to survive, slow growth of tissue culture seedlings, asexual reproduction, etc.

Inactive Publication Date: 2016-10-26
河南红枫生物高科股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The germination rate of Euonymus shaanxiensis seeds is extremely low under natural conditions. Conventional sowing experiments have proved that the germination rate is almost zero. 10%
Due to the limitation of seed germination conditions, most of the rapid propagation techniques of Euonymus euonymus in Shaanxi use the budded stem as explants, but the growth of tissue culture seedlings is slow, and hardening seedlings is not easy to survive.
At present, silk cotton wood is often used as rootstock for grafting and propagation in gardens. Although large-scale propagation of Euonymus shaanxi can be realized, grafting belongs to asexual reproduction, and gene recombination and variety improvement of euonymus shaanxi cannot be realized.

Method used

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  • Germinating method of Euonymus schensianus seeds
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  • Germinating method of Euonymus schensianus seeds

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Experimental program
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Embodiment 1

[0045] The embodiment of the present invention Euonymus shaanxi seed germination method is as follows:

[0046] 1. Seed treatment

[0047] (1) Between September 11 and October 1, select the Euonymus euonymus fruit with plump fruit, bluish-white peel, complete appearance, and no pests and diseases for collection.

[0048] (2) After harvesting, first soak and wash with clean water with detergent to remove insect eggs and stains left on the peel, then rinse with running water for 2 hours, and dry the water. Use a razor blade to cut off the fruit wing, upper fruit pedicle and lower navel without damaging the inner seeds.

[0049] (3) After cutting the seeds, soak them in 0.1% HClO for 10 minutes.

[0050] (4) Sterilize with 75% alcohol and 0.1% HgCl in sequence for 5 minutes in the ultra-clean workbench. Note: The number of uses of the disinfectant should not exceed 10 times, and it should be discarded and reconstituted after reaching the limit. Among them, alcohol is required...

Embodiment 2

[0067] The embodiment of the present invention Euonymus shaanxi seed germination method is as follows:

[0068] 2. Seed treatment

[0069] (1) Between September 11 and October 1, select the Euonymus euonymus fruit with plump fruit, bluish-white peel, complete appearance, and no pests and diseases for collection.

[0070] (2) After harvesting, first soak and wash with clean water with detergent to remove insect eggs and stains left on the peel, then rinse with running water for 2 hours, and dry the water. Use a razor blade to cut off the fruit wing, upper fruit pedicle and lower navel without damaging the inner seeds.

[0071] (3) After cutting the seeds, soak them in 0.1% HClO for 10 minutes.

[0072] (4) Sterilize with 75% alcohol and 0.1% HgCl in sequence for 5 minutes in the ultra-clean workbench. Note: The number of uses of the disinfectant should not exceed 10 times, and it should be discarded and reconstituted after reaching the limit. Among them, alcohol is required...

Embodiment 3

[0088] The embodiment of the present invention Euonymus shaanxi seed germination method is as follows:

[0089] 3. Seed treatment

[0090] (1) Between September 11 and October 1, select the Euonymus euonymus fruit with plump fruit, bluish-white peel, complete appearance, and no pests and diseases for collection.

[0091] (2) After harvesting, first soak and wash with clean water with detergent to remove insect eggs and stains left on the peel, then rinse with running water for 2 hours, and dry the water. Use a razor blade to cut off the fruit wing, upper fruit pedicle and lower navel without damaging the inner seeds.

[0092] (3) After cutting the seeds, soak them in 0.1% HClO for 10 minutes.

[0093] (4) Sterilize with 75% alcohol and 0.1% HgCl in sequence for 5 minutes in the ultra-clean workbench. Note: The number of uses of the disinfectant should not exceed 10 times, and it should be discarded and reconstituted after reaching the limit. Among them, alcohol is required...

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Abstract

The invention relates to technical field of agriculture, in particular to a germinating method of Euonymus schensianus seeds. The germinating method includes: sterilizing the Euonymus schensianus seeds, and cutting off part of endosperm away from hilum ends; performing rooting culture, to be more specific, inoculating the seeds with the endosperm being cut off into a rooting culture medium for culturing, and inoculating the rooting seeds into a seedling-hardening culture medium for culturing, wherein the rooting culture medium includes KT and NAA. The germinating method has the advantages that the method is used for inducing the germination of the Euonymus schensianus seeds, the germinating rate of the Euonymus schensianus seeds is increased from less than 10% to more than 90%, the seedling-hardening survival rate is about 85%, and the method is quite significant to the large-scale seed propagation of Euonymus schensianus.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a germination method of euonymus shaanxiensis seeds. Background technique [0002] Euonymus schensianus Maxim, also known as the golden silk hanging butterfly, is a samara of the genus Euonymus schensianus in the family Euonymus schensianus. The fruit has 4 to 5 wings and turns red when ripe. The wings can reach 2.5 to 3 cm in length and 1.5 to 2 cm in width. , hanging between the green leaves, like a butterfly fluttering its wings, is a tree species with high ornamental value in gardens. [0003] The propagation methods of Euonymus shaanxi include seeding and grafting. The sowing method is as follows: harvest the fruits after they are ripe in autumn, dry them in the sun, and collect the seeds after the peels are cracked, dry them, and store them. In January of the following year, soak the seeds in warm water at 30°C for 24 hours, and then store them in the shade of wet s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丹张茂张家勋王东超
Owner 河南红枫生物高科股份有限公司
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