Capillary electrophoresis detection system and method

Abstract

The invention provides a capillary electrophoresis detection system and method. At least two lasers and one capillary are provided. A detection sample in each capillary includes various target matters to be detected that include biological matters labeled with fluorescent dye or biological matters combined with an antibody labeled with fluorescent dye; a plurality of detectors are provided; laser that the at least two lasers emit irradiate the capillaries, the fluorescent dyes in the detection samples in the capillaries are excited by the laser to generate fluorescence of various wavelength ranges, the multiple detectors receive the fluorescence of various wavelength ranges, and the type and content of multiple target matters to be detected in the detection samples in the capillaries are detected. At least two lasers are used to irradiate capillaries and emit laser of different wavelengths, more wavelength ranges of fluorescence can be excited than that by a single laser, detecting speed is increased, and detection samples are saved.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a capillary electrophoresis detection system and detection method. Background technique [0002] Capillary electrophoresis (capillary electrophoresis, CE) technology is a new type of liquid phase separation technology with capillary as the separation channel and high voltage direct current electric field as the driving force. Capillary electrophoresis generally uses a quartz capillary column. Under the action of a high-voltage electric field outside the capillary, the buffer on one side moves toward the negative electrode due to its positive charge. At the same time, in the buffer solution, the charged biomolecules move towards the electrode with the opposite polarity to the charged biomolecules at different speeds under the action of the high-voltage electric field outside the capillary, forming electrophoresis. [0003] In the prior art, the capillary electrophoresis techniq...

AI Technical Summary

Problems solved by technology

If there are a large number of target substances to be detected in the detection sample, it can only be divided into multiple detections, the detection speed is slow, and the detection sample costs a lot

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