A Locking System of Microscope Lens and Sample Based on 4pi Microscope Architecture

Abstract

The invention discloses a microscopic lens and sample locking system based on a 4Pi microscope framework. The system comprises a laser device, a first lens, a first polarization beam splitter, a second polarization beam splitter, a first dichroscope, an upper microscopic lens, a sample stage, a lower microscopic lens, a second dichroscope, a second lens, a cylindrical lens, a first CMOS camera, an eccentric aperture diaphragm, a first reflecting mirror, a third lens, a second reflecting mirror and a second CMOS camera. The original framework of a 4Pi microscope is not changed, and the focusing locking system is introduced in the whole 4Pi system by utilizing the dichroscopes so that real-time locking of the relative position of the double lenses and the relative position of the lenses and the sample can be realized, and the whole focusing locking system is quite compact. Difference caused by separate imaging of the upper and lower lenses in the 4Pi microscopic system can be overcome so that the problem of image distortion caused by middle and later image processing of the conventional 4Pi microscope can be avoided and the working precision is high.

Description

technical field [0001] The invention relates to the field of optical instruments and the field of biomedical microscopic imaging, in particular to a microscope head and a sample locking system based on a 4Pi microscope architecture. Background technique [0002] At present, research in the field of biomedicine requires higher and higher resolution. Researchers need to understand the three-dimensional structure information of various sub-hundred nanometer-scale substances. However, traditional optical microscopes cannot achieve such resolution, especially in The resolving power in the optical axis direction is far from insufficient. Although electron microscopes and atomic force microscopes can provide higher resolution, they can only provide surface images and cannot image deep tissues and living cells. The emergence of the 4Pi confocal system perfectly solves this problem. According to Rayleigh data, increasing the numerical aperture of the objective lens can reduce the si...

AI Technical Summary

Problems solved by technology

[0004] Aiming at many problems in the existing 4Pi microscopic system, such as the inability to guarantee the relative position of the two lenses, the low precision, and the easy distortion of the later image processing, the present invention proposes a new microscopic lens and sample locking system based on the 4Pi architecture

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