Cerebral echinococcosis marker nad5 gene, and detection method and kit of Taenia multiceps

Abstract

The invention belongs to the field of animal disease diagnosis, and concretely relates to a cerebral echinococcosis marker nad5 gene, and a detection method and a kit of Taenia multiceps. A Taenia multiceps imago sample is collected in a laboratory, DNA is extracted from dog night soil, and PCR amplification is carried out by using parts of the sequence of the mitochondrial nad5 gene as the target DNA fragment of Taenia multiceps. A result shows that the length of the amplified target fragment of Taenia multiceps infected dog night soil is consistent with the length of an expected fragment, and is 433bp. A sequencing identification result shows that the homology of the Cerebral echinococcosis marker nad5 gene to parts of the sequence of the nad5 gene of a known Taenia multiceps strain is 99.5%. The nad5 gene is highly specific and sensitive. The nad5 gene can be used as a Cerebral echinococcosis marker. The detection method of Taenia multiceps can rapidly and effectively detect Taenia multiceps eggs in dog night soil, also has high specificity and sensitivity, and can be used for the epidemiology investigation of the Taenia multiceps.

Description

technical field [0001] The invention belongs to the field of animal disease diagnosis, and in particular relates to a detection method and a kit for a cerebral echinococcosis marker nad5 gene and a tapeworm. Background technique [0002] Taenia multiceps (Taenia multiceps) is a common tapeworm in the small intestine of dogs. Its gestational segments and eggs are often excreted with dog feces, and after being swallowed by herbivores such as sheep and cattle, they enter the body and develop into brain polycephales, causing cerebral hydatid. sick. Cerebral echinococcosis not only causes the death of livestock such as sheep and cattle, and causes huge economic losses to the breeding industry, but also threatens human health. Dogs infected with Taenia polycephala are an important source of infection for cerebral echinococcosis. Therefore, timely and accurately grasp the infection situation of Taenia polycephala in endemic areas and control the infection of dogs, cut off the tran...

AI Technical Summary

Problems solved by technology

Although the feces examination of animals is still the main method for epidemiological investigations, it is difficult to identify the species by morphological observation because the eggs of Taeniaceae are very similar in shape under the microscope, and the sensitivity of this method is not high, and it is easy to miss check

However, there is no simple method that can be popularized and applied in the monitoring of Taenia canis infection. Therefore, exploring a simple, fast and effective detection method for Taenia canis infection is of great importance to cut off the brain echinococcosis from the source. Popularity is very important

Images