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Application of lactobacillus rhamnosus grx19 in regulation of intestinal floras

A technology for regulating intestinal flora and Lactobacillus rhamnosus, which is applied in the direction of Lactobacillus, application, medical preparations containing active ingredients, etc., can solve the problems of other undisclosed applications, achieve excellent intestinal colonization ability, comprehensive Excellent ability and the effect of regulating the structure of intestinal flora

Inactive Publication Date: 2016-09-07
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The previous Chinese patent application "Lactobacillus rhamnosus grx19 and its application" (Application No.: 2011104416667) disclosed a strain of Lactobacillus rhamnosus grx19 (CGMCC No.5519) and its use in the preparation of live bacteria with long shelf life Type lactic acid bacteria in beverages and fermented milk products, but other applications of the bacteria are not disclosed

Method used

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  • Application of lactobacillus rhamnosus grx19 in regulation of intestinal floras
  • Application of lactobacillus rhamnosus grx19 in regulation of intestinal floras
  • Application of lactobacillus rhamnosus grx19 in regulation of intestinal floras

Examples

Experimental program
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Effect test

Embodiment 1

[0088] Example 1: Preparation of fermented milk using Lactobacillus rhamnosus grx19

[0089] The activated lactic acid bacteria grx19 culture for 2 generations was inoculated into reconstituted skimmed milk (10%) heat-treated at 95℃ / 5min at 3% inoculum, cultivated at 37℃ for 10h, and cooled at 4℃ to prepare starter. The number of viable bacteria is about 10 8 cfu / mL.

[0090] The reconstituted whole milk (11.5%) is heated to about 50°C, 6% sucrose is added, and after full dissolution, the reconstituted whole milk is preheated to about 60°C, and homogenized under a pressure of 20MPa. Then heat the reconstituted whole milk at 95℃ / 5min. After cooling to 38℃, add lactic acid bacteria grx19 starter at 5% inoculum, ferment to curd at 37℃, and store it at 4℃ after cooling. A fermented milk with high probiotic lactic acid bacteria adhesion is obtained.

Embodiment 2

[0091] Example 2: Preparation of fermented milk beverage using Lactobacillus rhamnosus grx19

[0092] 42kg of skimmed milk powder was dissolved in water at 50℃ to prepare 350kg of reconstituted skim milk. After being fully dissolved, the reconstituted skim milk was heat-treated at 95℃ / 5min. After it was cooled to 37℃, the inoculation amount was 5%. Add lactic acid bacteria grx19 starter, ferment for about 24h at 37℃, control the end point acidity at about 160°T, add 650kg of sugar and stabilizer mixed solution sterilized at 110℃ / 5s (the composition of the mixed solution is 12% sucrose, 0.1 % Monoglyceride, 0.1% sucrose ester and 0.4% pectin), after mixing, preheat to 60℃, homogenize under 20MPa pressure, then heat treatment at 75℃ / 15s, and cool to about 15℃. Bacteria filling, that is 1000kg of Lactobacillus rhamnosus grx19 fermented milk beverage.

Embodiment 3

[0093] Example 3: Preparation of lyophilized powder of probiotic lactic acid bacteria using Lactobacillus rhamnosus grx19

[0094] After fully mixing skimmed milk powder, oligofructose, yeast powder, peptone and purified water according to mass percentages of 12.0%, 2.0%, 0.8%, 0.7%, and 84.5%, preheated to 60°C and homogenized under 20MPa pressure. Then heat treatment at 95°C / 30min and cool to 37°C. The grx19 starter prepared in Example 1 was inoculated at 5% of the inoculum, cultured at 37°C for 10 hours, centrifuged, and freeze-dried in vacuum until the water content was less than 3% to obtain Lactobacillus rhamnosus grx19 freeze-dried powder.

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Abstract

The invention relates to new application of lactobacillus rhamnosus grx19, and discloses application of the lactobacillus rhamnosus in preparation of functional foods and drugs for regulating intestinal floras and preventing viral diarrheas. The lactic acid bacteria grx19 disclosed by the invention has good acid and bile salt tolerance and pathogenic bacteria inhibition, higher surface hydrophobicity, higher self-aggregation ability, and obvious adhesion for small intestinal epithelial crypt cells (IEC-6 cells) of rats shown by cell research. The lactobacillus grx19 disclosed by the invention has the effects beneficial to human health like general lactobacilli, and further can regulate the structures of the intestinal floras and prevent the viral diarrheas, thereby being used for production of the functional foods or drugs.

Description

Technical field [0001] The present invention relates to the field of food biotechnology, in particular to a strain of probiotic lactic acid bacteria capable of regulating the structure of intestinal flora and preventing viral diarrhea and its application in functional foods. Background technique [0002] The adhesion of lactic acid bacteria helps maintain the structure of the normal intestinal flora, maintain the integrity of the morphology and function of the intestinal mucosa, and prevent viral diarrhea. In maintaining the structure of the normal intestinal flora, lactic acid bacteria are mainly achieved through steric hindrance. When the highly adherent lactic acid bacteria colonize the intestinal tract before the pathogenic bacteria, a physical barrier can be formed on the surface of the intestinal tract, which blocks the contact of the pathogenic bacteria with the intestinal mucosa and facilitates the elimination of the pathogenic bacteria. In addition, high-adhesion probio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P1/12A23L33/135A23C9/123
CPCA23C9/1234A61K35/747A23V2002/00A23V2400/175A23V2200/32
Inventor 顾瑞霞瞿恒贤刘东方薛梅黄玉军陈霞陈大卫印佰星蒋欣荣瓦云超孟茜
Owner YANGZHOU UNIV
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