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A method for efficiently propagating Drosophila magna using the rice moth pupae as a host

A technology of rice moth pupae and leg wasp, which is applied in animal husbandry and other fields to achieve the effect of increasing the parasitism rate, eliminating cumbersome steps, and reducing the possibility of disease susceptibility

Active Publication Date: 2017-02-08
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no relevant report on the large-scale reproduction of this parasitic wasp in China

Method used

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  • A method for efficiently propagating Drosophila magna using the rice moth pupae as a host
  • A method for efficiently propagating Drosophila magna using the rice moth pupae as a host
  • A method for efficiently propagating Drosophila magna using the rice moth pupae as a host

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Raising instead of the host: spread a layer of feed with a thickness of 1 cm in the plastic box (the feed ingredients are calculated in parts by weight from 50 parts of corn flour, 25 parts of flour, 20 parts of wheat bran, 3 parts of white sugar, and 2 parts of yeast. ); evenly sprinkle 1mL rice moth eggs on the feed, and then use a sieve to sprinkle a layer of feed that just covers the eggs; after 15 days, feed once every 3 days, and the amount of input is the food intake of larvae for 3 days; after larvae develop to 30 days, , spread two parallel nylon ropes on the surface of the feed, with both ends of the ropes exposed, and then bury the ropes in the feed when feeding; when the larvae cocoon and pupate, put the ropes together with the cocoon block Lift them together; take the rice moth pupae with cocoons on the rope and set aside;

[0035] (2) Prepare numerous honeycomb cages: as figure 1 As shown, in the numerous bee chambers, the numerous bee cages 3 are pla...

Embodiment 2

[0046] (1) Raising instead of the host: spread a layer of 1.5cm thick feed in a plastic box (the feed ingredients are calculated by weight by 50 parts of corn flour, 25 parts of flour, 20 parts of wheat bran, 3 parts of white sugar, and 2 parts of yeast. Sprinkle 1.5mL rice moth eggs evenly on the feed, and then use a sieve to sprinkle a layer of feed that just covers the eggs; after 15 days, feed once every 3 days, and the input amount is the food intake of larvae for 3 days; After 30 days, spread two parallel nylon ropes on the surface of the feed, and the two ends of the ropes are exposed, and when feeding thereafter, the ropes are buried in the material; Lift the cocoon block together; take the rice moth pupae with cocoons on the rope and set aside;

[0047] (2) Prepare numerous honeycomb cages: as figure 1 As shown, in the numerous bee chambers, the numerous bee cages 3 are placed on the insect racks 2 provided with lamp tubes 1 and barbed wire 5 at the top, and the nume...

Embodiment 3

[0058] (1) Raising instead of the host: spread a layer of 1-1.5 cm thick feed in a plastic box (the feed ingredients are calculated by weight by 50 parts of corn flour, 25 parts of flour, 20 parts of wheat bran, 3 parts of white sugar, and 2 parts of yeast. Sprinkle 1.3mL rice moth eggs evenly on the feed, and then use a sieve to sprinkle a layer of feed that just covers the eggs; after 15 days, feed once every 3 days, and the input amount is the food intake of larvae for 3 days; After 30 days of development, spread two parallel nylon ropes on the surface of the feed, and the two ends of the ropes are exposed, and when feeding thereafter, the ropes are buried in the material; when the larvae cocoon and pupate, the The rope is lifted together with the cocoon block; take the cocooned rice moth chrysalis on the rope and set aside;

[0059] (2) Prepare numerous honeycomb cages: as figure 1 As shown, in the numerous bee chambers, the numerous bee cages 3 are placed on the insect r...

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Abstract

The invention discloses a method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts. According to the method, the cocooned corcyra cephalonica pupae which are refrigerated for 1.5 h at 5 DEG C are mainly taken as substitute hosts of the brachymeria lasus, the brachymeria lasus reproducing method is easy, convenient and rapid to oeprate, and the reproducing efficiency is high. Brachymeria lasus inoculation is conducted on the upper layer of a brachymeria lasus reproducing cage through the cocooned corcyra cephalonica pupae, the phototaxis of the brachymeria lasus is effectively utilized, the distance between the brachymeria lasus and a nutrient source and the hosts is shortened, the parasitism rate of the brachymeria lasus to the hosts and the feeding frequency are increased, the brachymeria lasus can be uninterruptedly and massively reproduced, therefore, scale production of the brachymeria lasus is achieved, and the obvious ecological benefit and economic benefit are achieved.

Description

technical field [0001] The invention relates to the field of biological control of agricultural and forestry pests, relates to a method for artificially raising parasitic wasps, and in particular to a method for efficiently multiplying the wasps by using rice moth pupae as hosts. Background technique [0002] Coconut weaving moth Opisina arenosella Walker, also known as black-headed crawler moth, coconut borer moth, and coconut wood moth, belongs to Lepidoptera Xylinidae. It is a serious damage to more than 30 kinds of palms such as coconut, palm palm, Middle East date palm, betel nut, and king palm. major pests of plants. The larvae mainly do damage from the old leaves of the lower layer of the coconut upwards and feed on the mesophyll. In severe cases, only 3 to 4 new leaves in the upper layer are left, and the rest of the leaves are eaten up, resulting in reduced production and quality. In severe cases, the output of coconut fruit is reduced by more than 45%. [0003] Co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 林玉英金涛彭正强金启安温海波
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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