A method of promoting napa cabbage microspore embryogeny and direct production of plantlets

Abstract

A method of promoting napa cabbage microspore embryogeny and direct production of plantlets is provided to overcome the problem that microspore culturing techniques at present are low in microspore embryogeny rate, low in the rate of direct production of plantlets and long in culturing period. Through adding a histone deacetylase inhibitor-trichostatin A (TSA) into an induction medium, the microspore embryogeny rate and the rate of direct production of plantlets of napa cabbage are increased. In an NLN induction medium to which 0.025-0.10 muM of the TSA is added, the microspore embryogeny rate of the napa cabbage is increased by 1.46-2.48 times, and the rate of direct production of plantlets of the napa cabbage is increased by 1.12-1.39 times. The medium is a liquid mixture consisting of the NLN induction medium, the 0.025-0.10 muM of the TSA, 130 g.L<-1> of cane sugar, 100 muL of sterilized agarose having a concentration of 0.5 g.L<-1> and 10 g.L<-1> of activated carbon.

Description

technical field [0001] The invention relates to a method for culturing free microspores of Chinese cabbage, belongs to the technical field of cell breeding, and is a method for promoting embryogenesis and direct seedling formation of Chinese cabbage microspores. Background technique [0002] Chinese cabbage (Brassica rapa. ssp. chinensis L.) is a typical cross-pollinated plant of Brassicaceae, which has significant heterosis. To take advantage of dominant hybrid breeding, the parents must be purified first. The traditional continuous selfing method takes a long time, which is labor-intensive and labor-intensive; it only takes one year to obtain homozygotes using the free microspore culture method, which saves time and saves time. The workload is reduced, and the regenerated plants cultivated in this way can not only create new breeding materials, but also have strong intergenerational stability and strong heterosis. In recent decades, some researchers at home and abroad hav...

AI Technical Summary

Problems solved by technology

To take advantage of dominant hybrid breeding, the parents must be purified first. The traditional continuous selfing method takes a long time, which is labor-intensive and labor-intensive; it only takes one year to obtain homozygotes using the free microspore culture method, which saves time and saves time. The workload is reduced, and the regenerated plants cultivated in this way can not only create new breeding materials, but also have strong intergenerational stability and strong heterosis

In recent decades, some researchers at home and abroad have done a lot of work on the cultivation of free microspores of cruciferous vegetables, and are committed to optimizing the system of microspore embryo formation and embryoid body seedling formation. However, there are still many genotypes of microspore embryogenesis. The low seedling rate and low embryoid body seedling rate limit the application range of this technology in actual breeding work

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