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Calix[4]arene adenine derivative-graphene oxide composite and its preparation method and application

A technology of adenine and derivatives, applied in the field of calix[4]arene adenine derivatives-graphene oxide composite materials and its preparation, to achieve the effects of improving sensitivity, facilitating large-scale industrial production, and simple and easy preparation process

Inactive Publication Date: 2019-02-01
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of metal complexes have been reported in the literature to detect the G-quadruplex of oncogene promoters, but there are no reports on the detection of G-quadruplexes using calixarene, a macrocyclic compound with a three-dimensional structure.

Method used

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  • Calix[4]arene adenine derivative-graphene oxide composite and its preparation method and application
  • Calix[4]arene adenine derivative-graphene oxide composite and its preparation method and application
  • Calix[4]arene adenine derivative-graphene oxide composite and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A calix[4]arene adenine derivative-graphene oxide composite (AC+GO), which is formed by connecting graphene oxide and a calix[4]arene adenine derivative through an amide bond.

[0036] The preparation method of the compound comprises the following steps:

[0037] (1) Weigh 100mg of graphene oxide, add 20mL of thionyl chloride and 1mL of DMF to the mixed solution, ultrasonically disperse it evenly, transfer it to a three-necked bottle after mixing, and react at 70°C for 48h, the reaction is over Afterwards, it was centrifuged and washed repeatedly with tetrahydrofuran, and freeze-dried to obtain 0.085 g of acyl-chlorinated graphene oxide powder.

[0038] (2) Dissolve excess calix[4]arene adenine derivatives with 50mL of DMF, add them into the acyl chlorided graphene oxide powder obtained in step (1), stir at room temperature for 36h, and obtain black particle suspension with good dispersibility solution, centrifuged, washed with saturated saline, centrifuged once with d...

Embodiment 2

[0088] A calix[4]arene adenine derivative-graphene oxide composite (AC+GO), which is formed by connecting graphene oxide and a calix[4]arene adenine derivative through an amide bond.

[0089] The preparation method of the compound comprises the following steps:

[0090] (1) Weigh 100mg of graphene oxide, add 20mL of thionyl chloride and 1mL of DMF to the mixed solution, ultrasonically disperse it evenly, transfer it to a three-necked bottle after mixing, and react at 60°C for 96h, the reaction is over After that, it was centrifuged and washed repeatedly with tetrahydrofuran, and freeze-dried to obtain acyl-chlorinated graphene oxide powder.

[0091] (2) Dissolve excess calix[4]arene adenine derivatives with 50mL of DMF, add them into the acyl chlorided graphene oxide powder obtained in step (1), stir at room temperature for 96h, and obtain black particle suspension with good dispersibility solution, centrifuged, washed with saturated saline, centrifuged once with distilled wa...

Embodiment 3

[0094] The compound of the present embodiment is basically the same as that of Example 1, the difference is that the preparation method of the compound of the present embodiment comprises the following steps:

[0095] (1) Weigh 100mg of graphene oxide, add 20mL of thionyl chloride and 1mL of DMF to the mixed solution, ultrasonically disperse it evenly, transfer it to a three-necked bottle after mixing and react at 80°C for 12h, and the reaction is over After that, it was centrifuged and washed repeatedly with tetrahydrofuran, and freeze-dried to obtain acyl-chlorinated graphene oxide powder.

[0096] (2) Dissolve excess calix[4]arene adenine derivatives with 50mL of DMF, add to the acyl chlorided graphene oxide powder obtained in step (1), stir at room temperature for 6h, and obtain black particle suspension with good dispersibility solution, centrifuged, washed with saturated saline, centrifuged once with distilled water, and dried to obtain a black powder product, namely the...

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Abstract

The invention relates to a calix[4]arene adenine derivative-oxidized graphene compound, a preparation method and applications thereof. The compound is formed by connecting oxidized graphene and calix[4]arene adenine derivatives through amido bonds. The preparation method comprises the following steps: (1) subjecting oxidized graphene to chloroformylation; (2) dissolving excess calix[4]arene adenine derivatives by an organic solvent, adding chloroformylated oxidized graphene powder, stirring for 6 to 96 hours at a room temperature to obtain a black particle suspension liquid, carrying out centrifugal separation, carrying out saturated salt water washing, adding distilled water, carrying out centrifugal separation, and drying to obtain black powder namely the calix[4]arene adenine derivative-oxidized graphene compound, which can be used to recognize, combine, and stabilize cancer gene promoter region G-quadrome. Compared with the prior art, the preparation technology is simple and easy to perform, and a novel method is provided to detect G-quadrome through biological macro-cycle molecules.

Description

technical field [0001] The invention relates to a calixarene derivative composite material, in particular to a calix[4]arene adenine derivative-graphene oxide composite material and a preparation method and application thereof. Background technique [0002] With the progress of society, the living environment of human beings has undergone tremendous changes today. Various pollution problems such as global warming, the hole in the ozone layer, the reduction of biodiversity, the spread of acid rain, the sharp decline of forests, and land desertification have followed. Various diseases have increased, and cancer is one of the major diseases that seriously endanger human health. Various anticancer drugs have increased accordingly, so it is an urgent problem for human beings to seek anticancer drugs with low toxicity, high efficiency and anticancer activity. [0003] The promoter is an important genetic region, a DNA sequence located in the upstream regulatory region of the 5' e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 王丽赵玉霞石硕
Owner TONGJI UNIV
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