Extraction technology of hijiki polysaccharide

A hijiki polysaccharide and extraction process technology, applied in the field of bioengineering, can solve the problems of low polysaccharide yield, long production time, and easily damaged polysaccharides, and achieve the effect of rich nutrition, simple equipment, and clear operating units

Active Publication Date: 2018-03-27
ANHUI KUNDA BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the various nutrients mainly composed of hijiki polysaccharides are water-soluble substances, so they can be extracted by hot water extraction; this extraction method is simple, feasible, easy to operate, and does not require high equipment, but its production The time is long, the polysaccharide yield is not high, and the waste is serious
The acid leaching method is easy to destroy the specific structure related to immune activity in polysaccharides, and both methods have their shortcomings

Method used

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  • Extraction technology of hijiki polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A process for extracting hijiki polysaccharides, the specific method steps are as follows:

[0039] (1) Pour 3000kg of water into the No. 1 multifunctional extraction tank and adjust the temperature to 90℃;

[0040] (2) Add 300kg of hijiki raw material to the No. 3 multifunctional extraction tank, start stirring and extraction, and the extraction time is 5 hours, during the process, keep the temperature between 85-90℃, the extraction is finished, sampling and testing the content of the extract;

[0041] (3) Discharge the extracted liquid in the No. 3 multifunctional extraction tank, pass through the vibrating screen, and enter the No. 2 multifunctional extraction tank. The temperature is maintained at 60-65℃, and the extracted liquid is 2200kg, which is 8 / 1000 by weight. Weigh cellulase;

[0042] (4) Add the cellulase directly to the No. 2 multifunctional extraction tank, stir for 30 minutes to dissolve it, then stir for 3-4 hours, and then let it stand for 14 hours for reactio...

Embodiment 2

[0051] A process for extracting hijiki polysaccharides, the specific method steps are as follows:

[0052] (1) Pour 3000kg of water into the No. 1 multifunctional extraction tank and adjust the temperature to 90℃;

[0053] (2) Add 300kg of hijiki raw material to the No. 3 multifunctional extraction tank, start stirring and extraction, and the extraction time is 5 hours, during the process, keep the temperature between 85-90℃, the extraction is finished, sampling and testing the content of the extract;

[0054] (3) Discharge the extracted liquid in the No. 3 multifunctional extraction tank, pass through the vibrating screen, and enter the No. 2 multifunctional extraction tank. The temperature is maintained at 60-65℃, and the extracted liquid is 2200kg, which is 8 / 1000 by weight. Weigh hemicellulase;

[0055] (4) Add the hemicellulase directly to the No. 2 multifunctional extraction tank, stir for 30 minutes to dissolve it, stir for 3-4 hours, and then let it stand for 14 hours to react...

Embodiment 3

[0064] A process for extracting hijiki polysaccharides, the specific method steps are as follows:

[0065] (1) Pour 3000kg of water into the No. 1 multifunctional extraction tank and adjust the temperature to 90℃;

[0066] (2) Add 300kg of hijiki raw material to the No. 3 multifunctional extraction tank, start stirring and extraction, and the extraction time is 5 hours, during the process, keep the temperature between 85-90℃, the extraction is finished, sampling and testing the content of the extract;

[0067] (3) Discharge the extracted liquid in the No. 3 multifunctional extraction tank, pass through the vibrating screen, and enter the No. 2 multifunctional extraction tank. The temperature is maintained at 60-65℃, and the extracted liquid is 2200kg, which is 8 / 1000 by weight. Weigh xylanase;

[0068] (4) Add xylanase directly to the No. 2 multifunctional extraction tank, stir for 30 minutes to dissolve it, then stir for 3-4 hours, and then let it stand for 14 hours to react. The enz...

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Abstract

A hijiki polysaccharide extraction process, 1) inject 3 tons of water into No. 1 tank; 2) add 300kg of raw materials to No. 3 tank, stir and extract; 3) release the extract in No. 3 tank and pour into No. 2 tank; 4 ) Add the enzyme directly to the No. 2 tank, dissolve it, and place it for 14 hours to be reacted; 5) Pour the liquid in the No. 2 tank into the No. 1 tank, stop when the liquid in the No. 1 tank is concentrated to 700kg, and start centrifugation The liquid after the centrifugation is put into the No. 2 tank, and after the centrifugation, start to concentrate and centrifuge for the second time; 6) the concentrated solution in the No. 2 tank is put into the decolorization tank, adjust the pH to 9 with ammonia, add hydrogen peroxide until it becomes Yellow; 7) After the decolorization is completed, put it into the alcohol precipitation tank, add ethanol, stir and let stand; 8) Filter the ethanol alcohol precipitation solution, add water to the filtered precipitate, redissolve in the deproteinization tank, and volatilize the ethanol; 9) The dissolved solution is filtered in a centrifuge, and the recovered supernatant is sent to the spray drying station; 10) Spray drying is carried out, and the content and protein of the product are detected.

Description

Technical field [0001] The invention relates to the technical field of bioengineering, in particular to a hijiki polysaccharide extraction process. Background technique [0002] Hijiki belongs to the genus Phaeophyta, Cyclades, Fucus, Sargassoceae, and Sargassum. The algae are yellow-brown, plump and juicy, 15 to 40 cm high, up to 2 meters or more. The algae body is composed of false roots, stems, leaves and air sacs. The false root is a sucker-shaped base retainer, and the stem is an upright cylindrical main branch, with leaves and airbags in the shape of serrations in the north; linear or rod-shaped in the south. The algae body is fresh yellowish brown, and the dry product is black. The leaves vary greatly and have many shapes. It grows on rocks in the low tide zone and is mostly distributed along the coast of my country, from the Liaodong Peninsula in the north to the Leizhou Peninsula in the south, with the most in the Zhejiang coast. Hijiki is recognized as the most edi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L29/30A23L33/105A23L33/00
CPCA23V2002/00C08B37/0003A23V2200/30A23V2250/21
Inventor 吴士斌
Owner ANHUI KUNDA BIOLOGICAL ENG TECH
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