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Sphaeroides phycobiliprotein extraction process

A technology of phycobiliprotein and extraction process of Gexianmi, which is applied in the fields of peptide preparation, decapeptide, organic chemistry, etc., and can solve the problems of few research reports of Gexianmi

Inactive Publication Date: 2016-07-06
ZHENGZHOU GUOSHOU BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the content of B vitamins in Gexianmi is relatively high, VB1 is 1.2mg / 100g, VB2 is 11.8mg / 100g, but there are few research reports on Gexianmi at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] The preparation method is as follows:

[0008] a. Take a dry sample of Gexianmi, add an appropriate amount of drinking water for dynamic buffering and stirring for half an hour; b. Take out the swollen Gexianmi, and freeze and thaw it repeatedly three times; c. Then take the frozen Gexianmi for machine Break the wall and smash it; d. Extract again (0.05mol / LK2HPO4 + NaCl solution with different concentrations); e. Refrigerate and centrifuge three times (4°C, 10000r / min, 30min); f. Dialyze for 24 hours; g. Finally Freeze-dry to obtain crude phycobiliprotein.

Embodiment 2

[0010] The preparation method is as follows:

[0011] a. Take a dry sample of Gexianmi, add an appropriate amount of drinking water to perform dynamic buffer stirring for half an hour; b. Take out the swollen Gexianmi, and repeat freezing and thawing four times; c. Then take the frozen Gexianmi The machine breaks the wall and smashes it; d, then extracts (0.05mol / LK2HPO4 + NaCl solution with different concentrations); e, performs refrigerated centrifugation four times (4°C, 20000r / min, 50min); f, and then performs dialysis for 30h; g , and finally freeze-drying to obtain crude phycobiliprotein.

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PUM

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Abstract

The invention relates to a sphaeroides phycobiliprotein extraction process. According to the invention, the sphaeroides phycobiliprotein extraction process and stability thereof are studied. The sphaeroides phycobiliprotein extraction process is optimized with single factor experiments. As a result, when NaCl concentration is 0.24mol / L, pH value is 7 and an extraction time is 4.2h, the phycobiliprotein yields are the highest, which are relatively 7.004%, 7.438%, and 5.942%. As a result of phycocyanin stability experiments, phycocyanin is relatively stable when the temperature is below 30 DEG C, an alcohol concentration is below 10% and the pH value is 6-8.

Description

technical field [0001] The invention relates to a process for extracting phycobiliprotein from kudzu rice. Background technique [0002] Gexianmi is a multicellular filamentous plant belonging to the genus Nostoc of the cyanophyta Nostocaceae. Its cell structure is simple, and the individual is composed of many spherical cells to form unbranched single-row filaments. Generally, the filaments are bead-shaped. , the group is spherical and visible to the naked eye. According to records, Gexianmi has the effects of improving eyesight and nourishing qi, clearing heat and detoxifying, eliminating fatigue, long-term food and prolonging life, and curing night blindness. The amino acid content of Gexianmi even exceeds the standard of FAO. In addition, the content of B vitamins in kudzu rice is relatively high, VB1 is 1.2mg / 100g, VB2 is 11.8mg / 100g, but there are few research reports on kudzu rice at home and abroad. [0003] Phycobiliproteins are a class of pigment complex protein...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/14
Inventor 王跃进
Owner ZHENGZHOU GUOSHOU BIOLOGICAL TECH CO LTD
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