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Application of sanguinarine hydrochloride to preparation of medicine for treating salmonella infection

A technology of salmonella and sanguinarine, which is applied in the field of medicine, can solve the problems of difficult treatment, prolonged bacterial excretion, and inability to shorten the course of a patient's disease.

Inactive Publication Date: 2016-07-06
JILIN UNIV
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  • Application Information

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Problems solved by technology

However, the application of antibacterial drugs cannot shorten the course of the patient's disease, but instead promotes the emergence of drug-resistant strains in the intestinal tract, prolongs the time for excreting bacteria, and causes difficulties in treatment

Method used

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  • Application of sanguinarine hydrochloride to preparation of medicine for treating salmonella infection
  • Application of sanguinarine hydrochloride to preparation of medicine for treating salmonella infection
  • Application of sanguinarine hydrochloride to preparation of medicine for treating salmonella infection

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Experimental program
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Embodiment Construction

[0014] 1. Detection test of Salmonella type III secretion system function

[0015] In this experiment, SL1344-TEM-SipA recombinant bacteria were constructed in vitro, sanguinarine hydrochloride and SL1344-TEM-SipA were co-incubated at 37°C and 180 rpm for 4 hours, Hela cells were used as carriers, and drug treatment groups were added to the cell culture at MOI=20 Add DMEM to the untreated bacterial solution, and add DMEM to the negative control, and infect for 30 minutes at 37°C. The cells were washed three times with PBS, and then CCF4 fluorescent substrate was added to observe the color change of the cells through a fluorescence microscope to detect the function of sanguinarine hydrochloride on the Salmonella type III secretion system. The experimental results showed that sanguinarine hydrochloride significantly inhibited the function of Salmonella type III secretion system. As can be seen from the flow cytometry, after sanguinarine hydrochloride treatment, the secretion am...

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Abstract

The invention relates to application of sanguinarine hydrochloride to preparation of medicine for treating salmonella infection. Human cervical carcinoma cells (hela) serve as an infection carrier, and it is verified through gentamicin protection experiments, in-vitro biochemical experiments and mouse salmonella infection models that sanguinarine hydrochloride has a remarkable inhibition effect on salmonella infection. In the process of treating salmonella infection through antibiotic, medicine resistance can be induced to be generated easily, and compared with antibiotic treatment, the function of salmonella type III secretory system can be directly inhibited by the traditional Chinese medicine compound sanguinarine hydrochloride, so that the anti-infection effect is achieved, medicine resistance cannot be induced to be generated easily, and the application of sanguinarine hydrochloride has the advantage of being high in cure rate.

Description

technical field [0001] The invention relates to the application of sanguinarine hydrochloride in the preparation of medicines for treating Salmonella infection, belonging to the field of medicine. Background technique [0002] Sanguinarine, mainly found in the whole plant of celandine, the root tuber of Viola, the whole plant of Boluohui, and the aboveground part of blood grass, has various pharmacological activities, such as antibacterial, insecticidal, improving liver function, enhance immunity, anti-tumor and other pharmacological effects. occupies an important position in clinical medicine. But at present, there is no report of sanguinarine hydrochloride being used for anti-salmonella infection both at home and abroad. [0003] Salmonella is one of the most important food-borne pathogens causing food poisoning in humans and animals, and it is also a zoonotic pathogen of great significance in global public health. Salmonella has a wide range of hosts in nature, most of...

Claims

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Application Information

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IPC IPC(8): A61K31/4741A61P31/04A61P1/00
CPCA61K31/4741Y02A50/30
Inventor 邓旭明张勇刘雁王建锋
Owner JILIN UNIV
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